Epidemiological studies have mainly focused on the potential health effects of total volume of individual behaviours. However, two persons may have the same volume of PA or SB but accumulated in a completely different sequence. The pattern of accumulating PA and SB might be more important for health effects than the total volume. Therefore, we developed a novel algorithm translating raw accelerometer data into detailed sequence maps defined by a combination of intensity and duration, considering how SB and PA across the intensity continuum are accumulated and alternated throughout the day and week.