4-Aminopyridine affects synaptosomal protein phosphorylation in rat hippocampal slices

Pierre N. E. de Graan, Loes H. Schrama, Arjen B. Brussaard, Reinhard Jork, Willem Hendrik Gispen

Research output: Contribution to journalArticleAcademicpeer-review


Rat brain hippocampal slices were incubated with or without the convulsant 4-aminopyridine (4-AP). From these slices a crude mitochondrial/synaptosomal membrane fraction was prepared and analyzed for endogenous protein phosphorylation. 4-AP (10-5 M) stimulated the phosphorylation of a 50 kDa protein by 86%. The phosphorylation of this 50 kDa protein is Ca2+/calmodulin-dependent and we suggest that this protein is the lower molecular weight subunit of Ca2+/calmodulin-dependent protein kinase II (CaMK II). © 1987.
Original languageEnglish
Pages (from-to)345-349
JournalBrain Research
Issue number1-2
Publication statusPublished - 24 Feb 1987
Externally publishedYes

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