TY - JOUR
T1 - 4-Aminopyridine affects synaptosomal protein phosphorylation in rat hippocampal slices
AU - de Graan, Pierre N. E.
AU - Schrama, Loes H.
AU - Brussaard, Arjen B.
AU - Jork, Reinhard
AU - Gispen, Willem Hendrik
PY - 1987/2/24
Y1 - 1987/2/24
N2 - Rat brain hippocampal slices were incubated with or without the convulsant 4-aminopyridine (4-AP). From these slices a crude mitochondrial/synaptosomal membrane fraction was prepared and analyzed for endogenous protein phosphorylation. 4-AP (10-5 M) stimulated the phosphorylation of a 50 kDa protein by 86%. The phosphorylation of this 50 kDa protein is Ca2+/calmodulin-dependent and we suggest that this protein is the lower molecular weight subunit of Ca2+/calmodulin-dependent protein kinase II (CaMK II). © 1987.
AB - Rat brain hippocampal slices were incubated with or without the convulsant 4-aminopyridine (4-AP). From these slices a crude mitochondrial/synaptosomal membrane fraction was prepared and analyzed for endogenous protein phosphorylation. 4-AP (10-5 M) stimulated the phosphorylation of a 50 kDa protein by 86%. The phosphorylation of this 50 kDa protein is Ca2+/calmodulin-dependent and we suggest that this protein is the lower molecular weight subunit of Ca2+/calmodulin-dependent protein kinase II (CaMK II). © 1987.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0023155583&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/3032338
U2 - 10.1016/0006-8993(87)91392-8
DO - 10.1016/0006-8993(87)91392-8
M3 - Article
C2 - 3032338
SN - 0006-8993
VL - 404
SP - 345
EP - 349
JO - Brain Research
JF - Brain Research
IS - 1-2
ER -