A 22-kd surface antigen detected by monoclonal antibody E 48 is exclusively expressed in stratified squamous and transitional epithelia

J. J. Quak, A. J.M. Balm, G. A.M.S. Van Dongen, J. G.P. Brakkee, R. J. Scheper, G. B. Snow, C. J.L.M. Meijer

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

After immunization of mice with viable cells of a metastasis of a laryngeal squamous cell carcinoma, a monoclonal antibody E 48 was obtained that detects an epitope present exclusively in squamous and transitional epithelium and their neoplastic counterparts. Immunoblotting revealed that E 48 recognizes a 22 kd molecule. Seventy-five of 76 squamous cell carcinomas from the head and neck, lung, cervix, and skin stained positively, whereas various adenocarcinomas from the colon, lung, and breast, and small cell lung carcinomas consistently stained negatively. The E 48 antigen, which is formaldehyde resistant, appears to be a reliable marker for differentiation of squamous cell carcinomas from adenocarcinoma, and small cell carcinomas.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalAmerican Journal of Pathology
Volume136
Issue number1
Publication statusPublished - 1 Jan 1990

Cite this

@article{6899418341fd4e56a3c81aac6a1ce382,
title = "A 22-kd surface antigen detected by monoclonal antibody E 48 is exclusively expressed in stratified squamous and transitional epithelia",
abstract = "After immunization of mice with viable cells of a metastasis of a laryngeal squamous cell carcinoma, a monoclonal antibody E 48 was obtained that detects an epitope present exclusively in squamous and transitional epithelium and their neoplastic counterparts. Immunoblotting revealed that E 48 recognizes a 22 kd molecule. Seventy-five of 76 squamous cell carcinomas from the head and neck, lung, cervix, and skin stained positively, whereas various adenocarcinomas from the colon, lung, and breast, and small cell lung carcinomas consistently stained negatively. The E 48 antigen, which is formaldehyde resistant, appears to be a reliable marker for differentiation of squamous cell carcinomas from adenocarcinoma, and small cell carcinomas.",
author = "Quak, {J. J.} and Balm, {A. J.M.} and {Van Dongen}, {G. A.M.S.} and Brakkee, {J. G.P.} and Scheper, {R. J.} and Snow, {G. B.} and Meijer, {C. J.L.M.}",
year = "1990",
month = "1",
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language = "English",
volume = "136",
pages = "191--197",
journal = "The American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
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}

A 22-kd surface antigen detected by monoclonal antibody E 48 is exclusively expressed in stratified squamous and transitional epithelia. / Quak, J. J.; Balm, A. J.M.; Van Dongen, G. A.M.S.; Brakkee, J. G.P.; Scheper, R. J.; Snow, G. B.; Meijer, C. J.L.M.

In: American Journal of Pathology, Vol. 136, No. 1, 01.01.1990, p. 191-197.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A 22-kd surface antigen detected by monoclonal antibody E 48 is exclusively expressed in stratified squamous and transitional epithelia

AU - Quak, J. J.

AU - Balm, A. J.M.

AU - Van Dongen, G. A.M.S.

AU - Brakkee, J. G.P.

AU - Scheper, R. J.

AU - Snow, G. B.

AU - Meijer, C. J.L.M.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - After immunization of mice with viable cells of a metastasis of a laryngeal squamous cell carcinoma, a monoclonal antibody E 48 was obtained that detects an epitope present exclusively in squamous and transitional epithelium and their neoplastic counterparts. Immunoblotting revealed that E 48 recognizes a 22 kd molecule. Seventy-five of 76 squamous cell carcinomas from the head and neck, lung, cervix, and skin stained positively, whereas various adenocarcinomas from the colon, lung, and breast, and small cell lung carcinomas consistently stained negatively. The E 48 antigen, which is formaldehyde resistant, appears to be a reliable marker for differentiation of squamous cell carcinomas from adenocarcinoma, and small cell carcinomas.

AB - After immunization of mice with viable cells of a metastasis of a laryngeal squamous cell carcinoma, a monoclonal antibody E 48 was obtained that detects an epitope present exclusively in squamous and transitional epithelium and their neoplastic counterparts. Immunoblotting revealed that E 48 recognizes a 22 kd molecule. Seventy-five of 76 squamous cell carcinomas from the head and neck, lung, cervix, and skin stained positively, whereas various adenocarcinomas from the colon, lung, and breast, and small cell lung carcinomas consistently stained negatively. The E 48 antigen, which is formaldehyde resistant, appears to be a reliable marker for differentiation of squamous cell carcinomas from adenocarcinoma, and small cell carcinomas.

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M3 - Article

VL - 136

SP - 191

EP - 197

JO - The American Journal of Pathology

JF - The American Journal of Pathology

SN - 0002-9440

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