A blaVIM-1 positive Aeromonas hydrophila strain in a near-drowning patient: Evidence for interspecies plasmid transfer within the patient

Thijs Bosch*, Rogier Schade, Fabian Landman, Leo Schouls, Karin Van Dijk

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Aim: To show that a strain of Aeromonas hydrophila became resistant to carbapenems by interspecies transfer of a plasmid using long-read sequencing. Material & methods: Whole genome sequencing of the four isolates was done using Illumina Hiseq, while the plasmid was reconstructed using the MinION sequencer. The resistome was identified with ResFinder. Results: Whole genome sequencing and long-read sequencing showed that all isolates carried a blaVIM-1 gene located on a 165 kb incA/C plasmid. ResFinder confirmed that the resistome of the plasmid, comprising 13 resistance genes, was identical within all isolates. Discussion: Long-read sequencing using the MinION successfully reconstructed a plasmid that was identical in all isolates, providing evidence for horizontal gene transfer of this blaVIM-1 gene carrying plasmid within the patient.

Original languageEnglish
Pages (from-to)1191-1197
Number of pages7
JournalFuture Microbiology
Volume14
Issue number14
DOIs
Publication statusPublished - 1 Jan 2019

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