A class II-restricted cytotoxic T-cell clone recognizes a human minor histocompatibility antigen with a restricted tissue distribution

Penny A. Holloway, Niels Kaldenhoven, Henriette M. Kok-Schoemaker, Mirjam Van Dijk, Hennie G. Otten, Marcel Tilanus, Saskia Postma, Tuna Mutis, Henk M. Lokhorst, Andries C. Bloem*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Following a human leucocyte antigen (HLA)-identical allogeneic stem cell transplantation (allo-SCT), minor histocompatibility antigens (mHags) play an important role in the induction of graft-versus-leukaemia (GvL) and graft-versus-myeloma (GvM). Many mHags show ubiquitous tissue expression and are associated with GvL and graft-versus-host disease. Here we describe a cytotoxic CD4+ T lymphocyte line and a cytotoxic, CD4+ T cell clone (CTC), 3AB11, which recognized a tissue-restricted mHag. This CTC was isolated from a multiple myeloma patient with clinical GvM following an HLA-matched allo-SCT. CTC 3AB11 was activated in a HLA-DP*0401 restricted fashion and the antigen was expressed by 27% of HLA-DP*0401 positive Epstein-Barr virus (EBV)-transformed B-cell lines (EBV-B). Tissue distribution analysis of antigen 3AB11 showed it to be expressed by patient-derived EBV-transformed B cell lines (EBVp), the myeloma plasma cell-line UM9 and monocytes. It was weakly expressed by peripheral blood-derived phytohaemagglutinin-induced T-cell blasts and absent on CD40L stimulated peripheral B (CD40L B) cells and stromal cells. The relatively high prevalence of the HLA class II-restricted 3AB11 antigen, together with its apparent haematopoietic-restricted expression, makes it an antigen of interest for cellular immunotherapy.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalBritish Journal of Haematology
Volume128
Issue number1
DOIs
Publication statusPublished - 1 Jan 2005

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