A Laser Microdissection-Liquid Chromatography-Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.

Original languageEnglish
Pages (from-to)371-383
Number of pages13
JournalMethods in Molecular Biology
Volume1723
DOIs
Publication statusPublished - 2018

Cite this

@article{8bdf25c594594470a9e240cf162c15d5,
title = "A Laser Microdissection-Liquid Chromatography-Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue",
abstract = "Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.",
keywords = "Journal Article",
author = "Hondius, {David C} and Hoozemans, {Jeroen J M} and Rozemuller, {Annemieke J M} and Li, {Ka Wan} and Smit, {August B}",
year = "2018",
doi = "10.1007/978-1-4939-7558-7_21",
language = "English",
volume = "1723",
pages = "371--383",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

A Laser Microdissection-Liquid Chromatography-Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue. / Hondius, David C; Hoozemans, Jeroen J M; Rozemuller, Annemieke J M; Li, Ka Wan; Smit, August B.

In: Methods in Molecular Biology, Vol. 1723, 2018, p. 371-383.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A Laser Microdissection-Liquid Chromatography-Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue

AU - Hondius, David C

AU - Hoozemans, Jeroen J M

AU - Rozemuller, Annemieke J M

AU - Li, Ka Wan

AU - Smit, August B

PY - 2018

Y1 - 2018

N2 - Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.

AB - Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.

KW - Journal Article

U2 - 10.1007/978-1-4939-7558-7_21

DO - 10.1007/978-1-4939-7558-7_21

M3 - Article

VL - 1723

SP - 371

EP - 383

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -