Abstract
Background Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods We determined levels of HMW and total adiponectin using assays by Lumipulse® and Millipore® respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) < 21 kg/m2, n = 25 elderly with BMI 30–35 kg/m2, n = 26 children). Results The Passing & Bablock regression analysis resulted in HMW adiponectinLUMIPULSE ∗ 0.5 − 0.9 = total adiponectinMILLIPORE, albeit with significant deviation from linearity (p < 0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition.
| Original language | English |
|---|---|
| Pages (from-to) | 30-33 |
| Number of pages | 4 |
| Journal | Clinica Chimica Acta |
| Volume | 465 |
| DOIs | |
| Publication status | Published - 1 Feb 2017 |
Cite this
}
A method comparison of total and HMW adiponectin : HMW/total adiponectin ratio varies versus total adiponectin, independent of clinical condition. / van Andel, Merel; Drent, Madeleine L.; van Herwaarden, Antonius E.; Ackermans, Mariëtte T.; Heijboer, Annemieke C.
In: Clinica Chimica Acta, Vol. 465, 01.02.2017, p. 30-33.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - A method comparison of total and HMW adiponectin
T2 - HMW/total adiponectin ratio varies versus total adiponectin, independent of clinical condition
AU - van Andel, Merel
AU - Drent, Madeleine L.
AU - van Herwaarden, Antonius E.
AU - Ackermans, Mariëtte T.
AU - Heijboer, Annemieke C.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Background Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods We determined levels of HMW and total adiponectin using assays by Lumipulse® and Millipore® respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) < 21 kg/m2, n = 25 elderly with BMI 30–35 kg/m2, n = 26 children). Results The Passing & Bablock regression analysis resulted in HMW adiponectinLUMIPULSE ∗ 0.5 − 0.9 = total adiponectinMILLIPORE, albeit with significant deviation from linearity (p < 0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition.
AB - Background Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods We determined levels of HMW and total adiponectin using assays by Lumipulse® and Millipore® respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) < 21 kg/m2, n = 25 elderly with BMI 30–35 kg/m2, n = 26 children). Results The Passing & Bablock regression analysis resulted in HMW adiponectinLUMIPULSE ∗ 0.5 − 0.9 = total adiponectinMILLIPORE, albeit with significant deviation from linearity (p < 0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition.
KW - Adiponectin isoforms
KW - Cardiovascular disease
KW - High molecular weight/total adiponectin ratio
KW - Method comparison
KW - Patient characteristics
UR - http://www.scopus.com/inward/record.url?scp=85006166031&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2016.12.009
DO - 10.1016/j.cca.2016.12.009
M3 - Article
VL - 465
SP - 30
EP - 33
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
ER -