A method comparison of total and HMW adiponectin: HMW/total adiponectin ratio varies versus total adiponectin, independent of clinical condition

Merel van Andel, Madeleine L. Drent, Antonius E. van Herwaarden, Mariëtte T. Ackermans, Annemieke C. Heijboer

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods We determined levels of HMW and total adiponectin using assays by Lumipulse® and Millipore® respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) < 21 kg/m2, n = 25 elderly with BMI 30–35 kg/m2, n = 26 children). Results The Passing & Bablock regression analysis resulted in HMW adiponectinLUMIPULSE ∗ 0.5 − 0.9 = total adiponectinMILLIPORE, albeit with significant deviation from linearity (p < 0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition.

Original languageEnglish
Pages (from-to)30-33
Number of pages4
JournalClinica Chimica Acta
Volume465
DOIs
Publication statusPublished - 1 Feb 2017

Cite this

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title = "A method comparison of total and HMW adiponectin: HMW/total adiponectin ratio varies versus total adiponectin, independent of clinical condition",
abstract = "Background Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods We determined levels of HMW and total adiponectin using assays by Lumipulse{\circledR} and Millipore{\circledR} respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) < 21 kg/m2, n = 25 elderly with BMI 30–35 kg/m2, n = 26 children). Results The Passing & Bablock regression analysis resulted in HMW adiponectinLUMIPULSE ∗ 0.5 − 0.9 = total adiponectinMILLIPORE, albeit with significant deviation from linearity (p < 0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition.",
keywords = "Adiponectin isoforms, Cardiovascular disease, High molecular weight/total adiponectin ratio, Method comparison, Patient characteristics",
author = "{van Andel}, Merel and Drent, {Madeleine L.} and {van Herwaarden}, {Antonius E.} and Ackermans, {Mari{\"e}tte T.} and Heijboer, {Annemieke C.}",
year = "2017",
month = "2",
day = "1",
doi = "10.1016/j.cca.2016.12.009",
language = "English",
volume = "465",
pages = "30--33",
journal = "Clinica Chimica Acta",
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A method comparison of total and HMW adiponectin : HMW/total adiponectin ratio varies versus total adiponectin, independent of clinical condition. / van Andel, Merel; Drent, Madeleine L.; van Herwaarden, Antonius E.; Ackermans, Mariëtte T.; Heijboer, Annemieke C.

In: Clinica Chimica Acta, Vol. 465, 01.02.2017, p. 30-33.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A method comparison of total and HMW adiponectin

T2 - HMW/total adiponectin ratio varies versus total adiponectin, independent of clinical condition

AU - van Andel, Merel

AU - Drent, Madeleine L.

AU - van Herwaarden, Antonius E.

AU - Ackermans, Mariëtte T.

AU - Heijboer, Annemieke C.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Background Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods We determined levels of HMW and total adiponectin using assays by Lumipulse® and Millipore® respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) < 21 kg/m2, n = 25 elderly with BMI 30–35 kg/m2, n = 26 children). Results The Passing & Bablock regression analysis resulted in HMW adiponectinLUMIPULSE ∗ 0.5 − 0.9 = total adiponectinMILLIPORE, albeit with significant deviation from linearity (p < 0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition.

AB - Background Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods We determined levels of HMW and total adiponectin using assays by Lumipulse® and Millipore® respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) < 21 kg/m2, n = 25 elderly with BMI 30–35 kg/m2, n = 26 children). Results The Passing & Bablock regression analysis resulted in HMW adiponectinLUMIPULSE ∗ 0.5 − 0.9 = total adiponectinMILLIPORE, albeit with significant deviation from linearity (p < 0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition.

KW - Adiponectin isoforms

KW - Cardiovascular disease

KW - High molecular weight/total adiponectin ratio

KW - Method comparison

KW - Patient characteristics

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U2 - 10.1016/j.cca.2016.12.009

DO - 10.1016/j.cca.2016.12.009

M3 - Article

VL - 465

SP - 30

EP - 33

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

ER -