A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system

M M Polfliet; P H Goede; E M van Kesteren-Hendrikx; N van Rooijen; C D Dijkstra; T K van den Berg

A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system

M M Polfliet, P H Goede, E M van Kesteren-Hendrikx, N van Rooijen, C D Dijkstra, T K van den Berg

Molecular cell biology and Immunology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The perivascular (PVM) and meningeal (MM) macrophages form a distinct population of resident CNS cells, selectively expressing the mature macrophage marker ED2 in the rat. In order to elucidate the role of the PVM and MM in rats during normal functioning of the brain and pathology, we have developed a strategy employing a single intraventricular injection of clodronate liposomes. This resulted in a complete depletion of the PVM and MM. Clodronate liposomes did not deplete the microglial cells. In other parts of the body, a temporal and mild depletion effect was observed, which was restored within 1 week. Detailed analysis of the elimination and repopulation kinetics of the PVM and MM revealed a slow repopulation of the CNS, starting at 14 days post depletion. This selective depletion method of the PVM and MM will enable us to get direct insight in their functions during normal and pathologic conditions of the CNS.

Original language	English
Pages (from-to)	188-95
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	116
Issue number	2
Publication status	Published - 1 Jun 2001

Cite this

@article{968df3167765435a9d6b0f7958ac4613,

title = "A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system",

abstract = "The perivascular (PVM) and meningeal (MM) macrophages form a distinct population of resident CNS cells, selectively expressing the mature macrophage marker ED2 in the rat. In order to elucidate the role of the PVM and MM in rats during normal functioning of the brain and pathology, we have developed a strategy employing a single intraventricular injection of clodronate liposomes. This resulted in a complete depletion of the PVM and MM. Clodronate liposomes did not deplete the microglial cells. In other parts of the body, a temporal and mild depletion effect was observed, which was restored within 1 week. Detailed analysis of the elimination and repopulation kinetics of the PVM and MM revealed a slow repopulation of the CNS, starting at 14 days post depletion. This selective depletion method of the PVM and MM will enable us to get direct insight in their functions during normal and pathologic conditions of the CNS.",

keywords = "Analgesics, Non-Narcotic/pharmacology, Animals, Blood-Brain Barrier/immunology, Cell Count, Central Nervous System/blood supply, Clodronic Acid/pharmacology, Kinetics, Liposomes/pharmacology, Macrophages/cytology, Male, Meninges/blood supply, Rats, Rats, Wistar",

author = "Polfliet, {M M} and Goede, {P H} and {van Kesteren-Hendrikx}, {E M} and {van Rooijen}, N and Dijkstra, {C D} and {van den Berg}, {T K}",

year = "2001",

month = jun,

day = "1",

language = "English",

volume = "116",

pages = "188--95",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system

AU - Polfliet, M M

AU - Goede, P H

AU - van Kesteren-Hendrikx, E M

AU - van Rooijen, N

AU - Dijkstra, C D

AU - van den Berg, T K

PY - 2001/6/1

Y1 - 2001/6/1

N2 - The perivascular (PVM) and meningeal (MM) macrophages form a distinct population of resident CNS cells, selectively expressing the mature macrophage marker ED2 in the rat. In order to elucidate the role of the PVM and MM in rats during normal functioning of the brain and pathology, we have developed a strategy employing a single intraventricular injection of clodronate liposomes. This resulted in a complete depletion of the PVM and MM. Clodronate liposomes did not deplete the microglial cells. In other parts of the body, a temporal and mild depletion effect was observed, which was restored within 1 week. Detailed analysis of the elimination and repopulation kinetics of the PVM and MM revealed a slow repopulation of the CNS, starting at 14 days post depletion. This selective depletion method of the PVM and MM will enable us to get direct insight in their functions during normal and pathologic conditions of the CNS.

AB - The perivascular (PVM) and meningeal (MM) macrophages form a distinct population of resident CNS cells, selectively expressing the mature macrophage marker ED2 in the rat. In order to elucidate the role of the PVM and MM in rats during normal functioning of the brain and pathology, we have developed a strategy employing a single intraventricular injection of clodronate liposomes. This resulted in a complete depletion of the PVM and MM. Clodronate liposomes did not deplete the microglial cells. In other parts of the body, a temporal and mild depletion effect was observed, which was restored within 1 week. Detailed analysis of the elimination and repopulation kinetics of the PVM and MM revealed a slow repopulation of the CNS, starting at 14 days post depletion. This selective depletion method of the PVM and MM will enable us to get direct insight in their functions during normal and pathologic conditions of the CNS.

KW - Analgesics, Non-Narcotic/pharmacology

KW - Animals

KW - Blood-Brain Barrier/immunology

KW - Cell Count

KW - Central Nervous System/blood supply

KW - Clodronic Acid/pharmacology

KW - Kinetics

KW - Liposomes/pharmacology

KW - Macrophages/cytology

KW - Male

KW - Meninges/blood supply

KW - Rats

KW - Rats, Wistar

M3 - Article

C2 - 11438173

SN - 0165-5728

VL - 116

SP - 188

EP - 195

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 2

ER -