A new porcine vascularized tibial bone allotransplantation model. Anatomy and surgical technique

Dimitra Kotsougiani, Joost I. Willems, Alexander Y. Shin, Patricia F. Friedrich, Caroline A. Hundepool, Allen T. Bishop

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Introduction: Vascularized bone allotransplantation may provide new options for reconstruction of segmental defects if problems of long-term immune modulation can be solved. The current literature lacks an orthotopic large animal model, limited to bone and without the confounding effects of other tissue types, permitting a multifaceted evaluation before new methods are used clinically. The purpose of this study was to develop a large animal model for vascularized bone allotransplantation. Materials and methods: Eight porcine hind limbs were dissected. Length, diameter, and location of all hindlimb vessels were measured and a single nutrient vessel supplying the tibial diaphysis identified enabling its use as a vascularized bone allotransplant. Four Yucatan minipigs were divided into two pairs with a major swine leukocyte antigen mismatch. A 3.5 cm tibial segment including its nutrient pedicle was raised simultaneously from each pig and transplanted into the matched defect of the other animal. Microarterial anastomosis of the pedicle and 3-drug immunosuppression maintained VCA viability. Bone healing and limb function were followed for 16 weeks. Results: A consistent tibia diaphyseal nutrient artery arose from the caudal tibial artery to enter bone a mean 2.8 mm distal to the tibial tubercle with a pedicle length of 6.6 ± 3.3 mm and diameter of 1.6 ± 0.2 mm. Using this pedicle, we reconstructed a 3.5 cm tibial defect with a vascularized bone allotransplant in four animals. Immediate weightbearing as well as progressive bone healing was demonstrated. Conclusion: We have developed a vascularized tibial bone allotranplantation large-animal model suitable for future bone-only allotranplantation research in mini-pigs.
Original languageEnglish
Pages (from-to)195-202
JournalMicrosurgery
Volume38
Issue number2
DOIs
Publication statusPublished - 2018

Cite this

Kotsougiani, D., Willems, J. I., Shin, A. Y., Friedrich, P. F., Hundepool, C. A., & Bishop, A. T. (2018). A new porcine vascularized tibial bone allotransplantation model. Anatomy and surgical technique. Microsurgery, 38(2), 195-202. https://doi.org/10.1002/micr.30255