A novel quantitative PCR assay for the detection of Streptococcus pneumoniae using the competence regulator gene target comX

Marrit N Habets, Amelieke J H Cremers, Martine P Bos, Paul Savelkoul, Marc J Eleveld, Jacques F Meis, Peter W M Hermans, Willem J Melchers, Marien I de Jonge, Dimitri A Diavatopoulos

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Streptococcus pneumoniae is responsible for an estimated 1.6 million deaths worldwide every year. While rapid detection and timely treatment with appropriate antibiotics is preferred, this is often difficult due to the amount of time that detection with blood cultures takes. In this study, a novel quantitative PCR assay for the detection of Streptococcus pneumoniae was developed. To identify novel targets, we analysed the pneumococcal genome for unique, repetitive DNA sequences. This approach identified comX, which is conserved and present in duplicate copies in Streptococcus pneumoniae but not in other bacterial species. Comparison with lytA, the current 'gold standard' for detection by quantitative PCR, demonstrated an analytic specificity of 100% for both assays on a panel of 10 pneumococcal and 18 non-pneumococcal isolates, but a reduction of 3.5 quantitation cycle values (± 0.23 sem), resulting in an increased analytical detection rate of comX. We validated our assay on DNA extracted from the serum of 30 bacteraemic patients who were blood culture positive for Streptococcus pneumoniae and 51 serum samples that were culture positive for other bacteria. This resulted in a similar clinical sensitivity between the comX and lytA assays (47%) and in a diagnostic specificity of 98.2 and 100% for the lytA and comX assays, respectively. In conclusion, we have developed a novel quantitative PCR assay with increased analytical sensitivity for the detection of Streptococcus pneumoniae, which may be used to develop a rapid bedside test for the direct detection of Streptococcus pneumoniae in clinical specimens.

Original languageEnglish
Pages (from-to)129-36
Number of pages8
JournalJournal of Medical Microbiology
Volume65
Issue number2
DOIs
Publication statusPublished - Feb 2016

Cite this

Habets, Marrit N ; Cremers, Amelieke J H ; Bos, Martine P ; Savelkoul, Paul ; Eleveld, Marc J ; Meis, Jacques F ; Hermans, Peter W M ; Melchers, Willem J ; de Jonge, Marien I ; Diavatopoulos, Dimitri A. / A novel quantitative PCR assay for the detection of Streptococcus pneumoniae using the competence regulator gene target comX. In: Journal of Medical Microbiology. 2016 ; Vol. 65, No. 2. pp. 129-36.
@article{7c428a65986e4943a6f1e75b63c38c33,
title = "A novel quantitative PCR assay for the detection of Streptococcus pneumoniae using the competence regulator gene target comX",
abstract = "Streptococcus pneumoniae is responsible for an estimated 1.6 million deaths worldwide every year. While rapid detection and timely treatment with appropriate antibiotics is preferred, this is often difficult due to the amount of time that detection with blood cultures takes. In this study, a novel quantitative PCR assay for the detection of Streptococcus pneumoniae was developed. To identify novel targets, we analysed the pneumococcal genome for unique, repetitive DNA sequences. This approach identified comX, which is conserved and present in duplicate copies in Streptococcus pneumoniae but not in other bacterial species. Comparison with lytA, the current 'gold standard' for detection by quantitative PCR, demonstrated an analytic specificity of 100{\%} for both assays on a panel of 10 pneumococcal and 18 non-pneumococcal isolates, but a reduction of 3.5 quantitation cycle values (± 0.23 sem), resulting in an increased analytical detection rate of comX. We validated our assay on DNA extracted from the serum of 30 bacteraemic patients who were blood culture positive for Streptococcus pneumoniae and 51 serum samples that were culture positive for other bacteria. This resulted in a similar clinical sensitivity between the comX and lytA assays (47{\%}) and in a diagnostic specificity of 98.2 and 100{\%} for the lytA and comX assays, respectively. In conclusion, we have developed a novel quantitative PCR assay with increased analytical sensitivity for the detection of Streptococcus pneumoniae, which may be used to develop a rapid bedside test for the direct detection of Streptococcus pneumoniae in clinical specimens.",
keywords = "Bacteremia, Bacterial Proteins, Female, Humans, Male, Pneumococcal Infections, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Streptococcus pneumoniae, Transcription Factors, Evaluation Studies, Journal Article",
author = "Habets, {Marrit N} and Cremers, {Amelieke J H} and Bos, {Martine P} and Paul Savelkoul and Eleveld, {Marc J} and Meis, {Jacques F} and Hermans, {Peter W M} and Melchers, {Willem J} and {de Jonge}, {Marien I} and Diavatopoulos, {Dimitri A}",
year = "2016",
month = "2",
doi = "10.1099/jmm.0.000204",
language = "English",
volume = "65",
pages = "129--36",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "2",

}

Habets, MN, Cremers, AJH, Bos, MP, Savelkoul, P, Eleveld, MJ, Meis, JF, Hermans, PWM, Melchers, WJ, de Jonge, MI & Diavatopoulos, DA 2016, 'A novel quantitative PCR assay for the detection of Streptococcus pneumoniae using the competence regulator gene target comX' Journal of Medical Microbiology, vol. 65, no. 2, pp. 129-36. https://doi.org/10.1099/jmm.0.000204

A novel quantitative PCR assay for the detection of Streptococcus pneumoniae using the competence regulator gene target comX. / Habets, Marrit N; Cremers, Amelieke J H; Bos, Martine P; Savelkoul, Paul; Eleveld, Marc J; Meis, Jacques F; Hermans, Peter W M; Melchers, Willem J; de Jonge, Marien I; Diavatopoulos, Dimitri A.

In: Journal of Medical Microbiology, Vol. 65, No. 2, 02.2016, p. 129-36.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A novel quantitative PCR assay for the detection of Streptococcus pneumoniae using the competence regulator gene target comX

AU - Habets, Marrit N

AU - Cremers, Amelieke J H

AU - Bos, Martine P

AU - Savelkoul, Paul

AU - Eleveld, Marc J

AU - Meis, Jacques F

AU - Hermans, Peter W M

AU - Melchers, Willem J

AU - de Jonge, Marien I

AU - Diavatopoulos, Dimitri A

PY - 2016/2

Y1 - 2016/2

N2 - Streptococcus pneumoniae is responsible for an estimated 1.6 million deaths worldwide every year. While rapid detection and timely treatment with appropriate antibiotics is preferred, this is often difficult due to the amount of time that detection with blood cultures takes. In this study, a novel quantitative PCR assay for the detection of Streptococcus pneumoniae was developed. To identify novel targets, we analysed the pneumococcal genome for unique, repetitive DNA sequences. This approach identified comX, which is conserved and present in duplicate copies in Streptococcus pneumoniae but not in other bacterial species. Comparison with lytA, the current 'gold standard' for detection by quantitative PCR, demonstrated an analytic specificity of 100% for both assays on a panel of 10 pneumococcal and 18 non-pneumococcal isolates, but a reduction of 3.5 quantitation cycle values (± 0.23 sem), resulting in an increased analytical detection rate of comX. We validated our assay on DNA extracted from the serum of 30 bacteraemic patients who were blood culture positive for Streptococcus pneumoniae and 51 serum samples that were culture positive for other bacteria. This resulted in a similar clinical sensitivity between the comX and lytA assays (47%) and in a diagnostic specificity of 98.2 and 100% for the lytA and comX assays, respectively. In conclusion, we have developed a novel quantitative PCR assay with increased analytical sensitivity for the detection of Streptococcus pneumoniae, which may be used to develop a rapid bedside test for the direct detection of Streptococcus pneumoniae in clinical specimens.

AB - Streptococcus pneumoniae is responsible for an estimated 1.6 million deaths worldwide every year. While rapid detection and timely treatment with appropriate antibiotics is preferred, this is often difficult due to the amount of time that detection with blood cultures takes. In this study, a novel quantitative PCR assay for the detection of Streptococcus pneumoniae was developed. To identify novel targets, we analysed the pneumococcal genome for unique, repetitive DNA sequences. This approach identified comX, which is conserved and present in duplicate copies in Streptococcus pneumoniae but not in other bacterial species. Comparison with lytA, the current 'gold standard' for detection by quantitative PCR, demonstrated an analytic specificity of 100% for both assays on a panel of 10 pneumococcal and 18 non-pneumococcal isolates, but a reduction of 3.5 quantitation cycle values (± 0.23 sem), resulting in an increased analytical detection rate of comX. We validated our assay on DNA extracted from the serum of 30 bacteraemic patients who were blood culture positive for Streptococcus pneumoniae and 51 serum samples that were culture positive for other bacteria. This resulted in a similar clinical sensitivity between the comX and lytA assays (47%) and in a diagnostic specificity of 98.2 and 100% for the lytA and comX assays, respectively. In conclusion, we have developed a novel quantitative PCR assay with increased analytical sensitivity for the detection of Streptococcus pneumoniae, which may be used to develop a rapid bedside test for the direct detection of Streptococcus pneumoniae in clinical specimens.

KW - Bacteremia

KW - Bacterial Proteins

KW - Female

KW - Humans

KW - Male

KW - Pneumococcal Infections

KW - Real-Time Polymerase Chain Reaction

KW - Sensitivity and Specificity

KW - Streptococcus pneumoniae

KW - Transcription Factors

KW - Evaluation Studies

KW - Journal Article

U2 - 10.1099/jmm.0.000204

DO - 10.1099/jmm.0.000204

M3 - Article

VL - 65

SP - 129

EP - 136

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - 2

ER -