A proteomics approach to identify long-term molecular changes in rat medial prefrontal cortex resulting from sucrose self-administration

Michel C Van den Oever, Sabine Spijker, Ka Wan Li, Connie R Jiménez, Eisuke Koya, Roel C. van der Schors, Yvonne Gouwenberg, Rob Binnekade, Taco J De Vries, Anton N M Schoffelmeer, August B. Smit

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The medial prefrontal cortex (mPFC) is involved in the processing and retrieval of reward-related information. Here, we investigated long-lasting changes in protein composition of the mPFC in rats with a history of sucrose self-administration. Protein levels were analyzed using 2-D PAGE and MALDI-TOF sequencing. From approximately 1500 spots, 28 regulated proteins were unambiguously identified and were involved in cytoskeleton organization, energy metabolism, oxidative stress, neurotransmission, and neuronal outgrowth and differentiation. For several proteins, this change was also found as a long-lasting alteration in gene expression. We show that self-administration of sucrose produces long-lasting molecular neuroadaptations in the mPFC that may be involved in reward-related information processing.

Original languageEnglish
Pages (from-to)147-54
Number of pages8
JournalJournal of Proteome Research
Issue number1
Publication statusPublished - Jan 2006

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