TY - JOUR
T1 - A two-gene methylation signature for the diagnosis of bladder cancer in urine
AU - Bosschieter, Judith
AU - Nieuwenhuijzen, Jakko A
AU - Hentschel, Anouk
AU - van Splunter, Annina P
AU - Segerink, Loes I
AU - Vis, André N
AU - Wilting, Saskia M
AU - Lissenberg-Witte, Birgit I
AU - A van Moorselaar, R Jeroen
AU - Steenbergen, Renske Dm
PY - 2019/2
Y1 - 2019/2
N2 - AIM: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine.PATIENTS & METHODS: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel.RESULTS: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86-99) and a specificity of 85% (95% CI: 76-94).CONCLUSION: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.
AB - AIM: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine.PATIENTS & METHODS: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel.RESULTS: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86-99) and a specificity of 85% (95% CI: 76-94).CONCLUSION: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.
KW - biomarkers
KW - methylation
KW - molecular diagnostics
KW - urinary bladder neoplasms
KW - urine analysis
UR - http://www.scopus.com/inward/record.url?scp=85061513673&partnerID=8YFLogxK
U2 - 10.2217/epi-2018-0094
DO - 10.2217/epi-2018-0094
M3 - Article
C2 - 30706728
VL - 11
SP - 337
EP - 347
JO - Epigenomics
JF - Epigenomics
SN - 1750-1911
IS - 3
ER -