A two-gene methylation signature for the diagnosis of bladder cancer in urine

Judith Bosschieter, Jakko A Nieuwenhuijzen, Anouk Hentschel, Annina P van Splunter, Loes I Segerink, André N Vis, Saskia M Wilting, Birgit I Lissenberg-Witte, R Jeroen A van Moorselaar, Renske Dm Steenbergen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

AIM: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine.

PATIENTS & METHODS: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel.

RESULTS: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86-99) and a specificity of 85% (95% CI: 76-94).

CONCLUSION: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.

Original languageEnglish
Pages (from-to)337-347
Number of pages11
JournalEpigenomics
Volume11
Issue number3
Early online date1 Feb 2019
DOIs
Publication statusPublished - Feb 2019

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