Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection

Karin Loré, Anders Sönnerborg, Christina Broström, Li-Ean Goh, Luc Perrin, Hugh McDade, Hans-Jürgen Stellbrink, Brian Gazzard, Rainer Weber, Laura A Napolitano, Yvette van Kooyk, Jan Andersson

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

BACKGROUND: Dendritic cells (DC) are target cells for HIV-1 and play a key role in antigen presentation and activation of T cells.

OBJECTIVE: To characterize interdigitating DC in lymphoid tissue (LT) with regard to maturation, expression of cytokines and co-stimulatory molecules in HIV-1-positive patients.

METHODS: DC were characterized by immunohistochemistry and in situ imaging in LT from patients with acute HIV-1 infection (aHI), antiretroviral treated patients, long-term non-progressors/slow progressors with HIV-1 infection (LTNP/SLP), patients with AIDS, HIV-1-negative controls and patients with acute Epstein-Barr virus (EBV) infection.

RESULTS: A significant increase of interdigitating DC expressing CD1a, S-100b, CD83 and DC-SIGN was found in LT from patients with aHI (P < 0.02). The co-stimulatory molecules CD80 and CD86 were, however, only partially upregulated and the complete parafollicular network found in acute EBV infection was not generated, despite increased expression of interleukins 1alpha, 1beta, 12; interleukin 1alpha receptor antagonist; interferon alpha; and CD40 expression. LTNP/SLP and treated aviremic subjects had increased frequency of interdigitating DC, albeit lower than in aHI, and low expression of CD80 and CD86. In contrast, patients with AIDS had fewer DC and reduced cytokine expression in LT.

CONCLUSIONS: In the early phase of HIV-1 infection, there was a migration of DC to LT comparable to that found in acute EBV infection. The infiltration of DC in LT in acute EBV infection was accompanied by upregulation of CD80 and CD86 expression, which did not occur in aHI. This co-stimulatory defect in aHI may have an impact on the development of HIV-1-specific T cell immunity.

Original languageEnglish
Pages (from-to)683-92
Number of pages10
JournalAIDS
Volume16
Issue number5
Publication statusPublished - 29 Mar 2002

Cite this

Loré, K., Sönnerborg, A., Broström, C., Goh, L-E., Perrin, L., McDade, H., ... Andersson, J. (2002). Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection. AIDS, 16(5), 683-92.
Loré, Karin ; Sönnerborg, Anders ; Broström, Christina ; Goh, Li-Ean ; Perrin, Luc ; McDade, Hugh ; Stellbrink, Hans-Jürgen ; Gazzard, Brian ; Weber, Rainer ; Napolitano, Laura A ; van Kooyk, Yvette ; Andersson, Jan. / Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection. In: AIDS. 2002 ; Vol. 16, No. 5. pp. 683-92.
@article{e5bdb1876a244852b11b15441b0c0ffc,
title = "Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection",
abstract = "BACKGROUND: Dendritic cells (DC) are target cells for HIV-1 and play a key role in antigen presentation and activation of T cells.OBJECTIVE: To characterize interdigitating DC in lymphoid tissue (LT) with regard to maturation, expression of cytokines and co-stimulatory molecules in HIV-1-positive patients.METHODS: DC were characterized by immunohistochemistry and in situ imaging in LT from patients with acute HIV-1 infection (aHI), antiretroviral treated patients, long-term non-progressors/slow progressors with HIV-1 infection (LTNP/SLP), patients with AIDS, HIV-1-negative controls and patients with acute Epstein-Barr virus (EBV) infection.RESULTS: A significant increase of interdigitating DC expressing CD1a, S-100b, CD83 and DC-SIGN was found in LT from patients with aHI (P < 0.02). The co-stimulatory molecules CD80 and CD86 were, however, only partially upregulated and the complete parafollicular network found in acute EBV infection was not generated, despite increased expression of interleukins 1alpha, 1beta, 12; interleukin 1alpha receptor antagonist; interferon alpha; and CD40 expression. LTNP/SLP and treated aviremic subjects had increased frequency of interdigitating DC, albeit lower than in aHI, and low expression of CD80 and CD86. In contrast, patients with AIDS had fewer DC and reduced cytokine expression in LT.CONCLUSIONS: In the early phase of HIV-1 infection, there was a migration of DC to LT comparable to that found in acute EBV infection. The infiltration of DC in LT in acute EBV infection was accompanied by upregulation of CD80 and CD86 expression, which did not occur in aHI. This co-stimulatory defect in aHI may have an impact on the development of HIV-1-specific T cell immunity.",
keywords = "Acute Disease, Antigens, CD/biosynthesis, B7-1 Antigen/biosynthesis, B7-2 Antigen, Biomarkers, CD40 Antigens/immunology, CD8 Antigens/biosynthesis, Cell Adhesion Molecules, Cytokines/biosynthesis, Dendritic Cells/immunology, HIV Infections/immunology, HIV-1/immunology, Lectins/immunology, Lectins, C-Type, Lymphoid Tissue/immunology, Membrane Glycoproteins/biosynthesis, Receptors, Cell Surface/immunology",
author = "Karin Lor{\'e} and Anders S{\"o}nnerborg and Christina Brostr{\"o}m and Li-Ean Goh and Luc Perrin and Hugh McDade and Hans-J{\"u}rgen Stellbrink and Brian Gazzard and Rainer Weber and Napolitano, {Laura A} and {van Kooyk}, Yvette and Jan Andersson",
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journal = "AIDS",
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Loré, K, Sönnerborg, A, Broström, C, Goh, L-E, Perrin, L, McDade, H, Stellbrink, H-J, Gazzard, B, Weber, R, Napolitano, LA, van Kooyk, Y & Andersson, J 2002, 'Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection' AIDS, vol. 16, no. 5, pp. 683-92.

Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection. / Loré, Karin; Sönnerborg, Anders; Broström, Christina; Goh, Li-Ean; Perrin, Luc; McDade, Hugh; Stellbrink, Hans-Jürgen; Gazzard, Brian; Weber, Rainer; Napolitano, Laura A; van Kooyk, Yvette; Andersson, Jan.

In: AIDS, Vol. 16, No. 5, 29.03.2002, p. 683-92.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection

AU - Loré, Karin

AU - Sönnerborg, Anders

AU - Broström, Christina

AU - Goh, Li-Ean

AU - Perrin, Luc

AU - McDade, Hugh

AU - Stellbrink, Hans-Jürgen

AU - Gazzard, Brian

AU - Weber, Rainer

AU - Napolitano, Laura A

AU - van Kooyk, Yvette

AU - Andersson, Jan

PY - 2002/3/29

Y1 - 2002/3/29

N2 - BACKGROUND: Dendritic cells (DC) are target cells for HIV-1 and play a key role in antigen presentation and activation of T cells.OBJECTIVE: To characterize interdigitating DC in lymphoid tissue (LT) with regard to maturation, expression of cytokines and co-stimulatory molecules in HIV-1-positive patients.METHODS: DC were characterized by immunohistochemistry and in situ imaging in LT from patients with acute HIV-1 infection (aHI), antiretroviral treated patients, long-term non-progressors/slow progressors with HIV-1 infection (LTNP/SLP), patients with AIDS, HIV-1-negative controls and patients with acute Epstein-Barr virus (EBV) infection.RESULTS: A significant increase of interdigitating DC expressing CD1a, S-100b, CD83 and DC-SIGN was found in LT from patients with aHI (P < 0.02). The co-stimulatory molecules CD80 and CD86 were, however, only partially upregulated and the complete parafollicular network found in acute EBV infection was not generated, despite increased expression of interleukins 1alpha, 1beta, 12; interleukin 1alpha receptor antagonist; interferon alpha; and CD40 expression. LTNP/SLP and treated aviremic subjects had increased frequency of interdigitating DC, albeit lower than in aHI, and low expression of CD80 and CD86. In contrast, patients with AIDS had fewer DC and reduced cytokine expression in LT.CONCLUSIONS: In the early phase of HIV-1 infection, there was a migration of DC to LT comparable to that found in acute EBV infection. The infiltration of DC in LT in acute EBV infection was accompanied by upregulation of CD80 and CD86 expression, which did not occur in aHI. This co-stimulatory defect in aHI may have an impact on the development of HIV-1-specific T cell immunity.

AB - BACKGROUND: Dendritic cells (DC) are target cells for HIV-1 and play a key role in antigen presentation and activation of T cells.OBJECTIVE: To characterize interdigitating DC in lymphoid tissue (LT) with regard to maturation, expression of cytokines and co-stimulatory molecules in HIV-1-positive patients.METHODS: DC were characterized by immunohistochemistry and in situ imaging in LT from patients with acute HIV-1 infection (aHI), antiretroviral treated patients, long-term non-progressors/slow progressors with HIV-1 infection (LTNP/SLP), patients with AIDS, HIV-1-negative controls and patients with acute Epstein-Barr virus (EBV) infection.RESULTS: A significant increase of interdigitating DC expressing CD1a, S-100b, CD83 and DC-SIGN was found in LT from patients with aHI (P < 0.02). The co-stimulatory molecules CD80 and CD86 were, however, only partially upregulated and the complete parafollicular network found in acute EBV infection was not generated, despite increased expression of interleukins 1alpha, 1beta, 12; interleukin 1alpha receptor antagonist; interferon alpha; and CD40 expression. LTNP/SLP and treated aviremic subjects had increased frequency of interdigitating DC, albeit lower than in aHI, and low expression of CD80 and CD86. In contrast, patients with AIDS had fewer DC and reduced cytokine expression in LT.CONCLUSIONS: In the early phase of HIV-1 infection, there was a migration of DC to LT comparable to that found in acute EBV infection. The infiltration of DC in LT in acute EBV infection was accompanied by upregulation of CD80 and CD86 expression, which did not occur in aHI. This co-stimulatory defect in aHI may have an impact on the development of HIV-1-specific T cell immunity.

KW - Acute Disease

KW - Antigens, CD/biosynthesis

KW - B7-1 Antigen/biosynthesis

KW - B7-2 Antigen

KW - Biomarkers

KW - CD40 Antigens/immunology

KW - CD8 Antigens/biosynthesis

KW - Cell Adhesion Molecules

KW - Cytokines/biosynthesis

KW - Dendritic Cells/immunology

KW - HIV Infections/immunology

KW - HIV-1/immunology

KW - Lectins/immunology

KW - Lectins, C-Type

KW - Lymphoid Tissue/immunology

KW - Membrane Glycoproteins/biosynthesis

KW - Receptors, Cell Surface/immunology

M3 - Article

VL - 16

SP - 683

EP - 692

JO - AIDS

JF - AIDS

SN - 0269-9370

IS - 5

ER -

Loré K, Sönnerborg A, Broström C, Goh L-E, Perrin L, McDade H et al. Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection. AIDS. 2002 Mar 29;16(5):683-92.