Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA

E van de Wiel-van Kemenade, Y van Kooyk, A J de Boer, R J Huijbens, P Weder, W van de Kasteele, C J Melief, C G Figdor

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Investigating the regulation of very late antigen (VLA)-mediated functions, we found that TS2/16, a mAb directed against the beta chain of the VLA group of integrins, can induce binding of resting peripheral blood lymphocytes, cloned T lymphocytes, and Epstein Barr virus-transformed B cells to extracellular matrix components, fibronectin, laminin, and collagen, but not to fibrinogen. The antibody stimulates VLA-4-, VLA-5-, and VLA-6-mediated binding. Furthermore, it induces VLA-4-mediated binding to vascular cell adhesion molecule-1 expressed by rTNF-alpha-stimulated endothelial cells, but it does not stimulate homotypic aggregation of cells as described for a number of anti-VLA-4 alpha antibodies (Bednarczyk, J.L., and B. W. McIntyre. 1990. J. Immunol. 144: 777-784; Campanero, M. R., R. Pulido, M. A. Ursa, M. Rodríguez-Moya, M. O. de Landázuri, and F. Sánchez-Madrid. 1990. J. Cell Biol. 110:2157-2165). Therefore, the stimulating activity of this anti-beta 1 antibody clearly contrasts with that of the anti-VLA-4 alpha antibodies, which induce homotypic cell aggregation, but not binding of cells to extracellular matrix components or endothelial cells, indicating that TS2/16 may generate different signals. The observation that also F(ab')2 or Fab fragments of this anti-beta 1 antibody stimulate binding to extracellular matrix components and endothelial cells excludes the possibility that binding requires receptor crosslinking, or is Fc receptor mediated. Induction of this adhesion is cation and energy dependent and requires an intact cytoskeleton. Although changes in the conformation of VLA integrins induced by this antibody may regulate their functional activity, the dependence on metabolic energy indicates that intracellular processes may also play a role.

Original languageEnglish
Pages (from-to)461-70
Number of pages10
JournalJournal of Cell Biology
Volume117
Issue number2
Publication statusPublished - Apr 1992

Cite this

van de Wiel-van Kemenade, E., van Kooyk, Y., de Boer, A. J., Huijbens, R. J., Weder, P., van de Kasteele, W., ... Figdor, C. G. (1992). Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA. Journal of Cell Biology, 117(2), 461-70.
van de Wiel-van Kemenade, E ; van Kooyk, Y ; de Boer, A J ; Huijbens, R J ; Weder, P ; van de Kasteele, W ; Melief, C J ; Figdor, C G. / Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA. In: Journal of Cell Biology. 1992 ; Vol. 117, No. 2. pp. 461-70.
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title = "Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA",
abstract = "Investigating the regulation of very late antigen (VLA)-mediated functions, we found that TS2/16, a mAb directed against the beta chain of the VLA group of integrins, can induce binding of resting peripheral blood lymphocytes, cloned T lymphocytes, and Epstein Barr virus-transformed B cells to extracellular matrix components, fibronectin, laminin, and collagen, but not to fibrinogen. The antibody stimulates VLA-4-, VLA-5-, and VLA-6-mediated binding. Furthermore, it induces VLA-4-mediated binding to vascular cell adhesion molecule-1 expressed by rTNF-alpha-stimulated endothelial cells, but it does not stimulate homotypic aggregation of cells as described for a number of anti-VLA-4 alpha antibodies (Bednarczyk, J.L., and B. W. McIntyre. 1990. J. Immunol. 144: 777-784; Campanero, M. R., R. Pulido, M. A. Ursa, M. Rodr{\'i}guez-Moya, M. O. de Land{\'a}zuri, and F. S{\'a}nchez-Madrid. 1990. J. Cell Biol. 110:2157-2165). Therefore, the stimulating activity of this anti-beta 1 antibody clearly contrasts with that of the anti-VLA-4 alpha antibodies, which induce homotypic cell aggregation, but not binding of cells to extracellular matrix components or endothelial cells, indicating that TS2/16 may generate different signals. The observation that also F(ab')2 or Fab fragments of this anti-beta 1 antibody stimulate binding to extracellular matrix components and endothelial cells excludes the possibility that binding requires receptor crosslinking, or is Fc receptor mediated. Induction of this adhesion is cation and energy dependent and requires an intact cytoskeleton. Although changes in the conformation of VLA integrins induced by this antibody may regulate their functional activity, the dependence on metabolic energy indicates that intracellular processes may also play a role.",
keywords = "Antibodies, Monoclonal/immunology, B-Lymphocytes/physiology, Cell Adhesion, Cell Aggregation, Cell Line, Cell Line, Transformed, Cells, Cultured, Collagen/metabolism, Endothelium/physiology, Extracellular Matrix/physiology, Fibrinogen/metabolism, Fibronectins/metabolism, Humans, Laminin/metabolism, Receptors, Very Late Antigen/immunology, T-Lymphocytes/physiology, Tetradecanoylphorbol Acetate/pharmacology",
author = "{van de Wiel-van Kemenade}, E and {van Kooyk}, Y and {de Boer}, {A J} and Huijbens, {R J} and P Weder and {van de Kasteele}, W and Melief, {C J} and Figdor, {C G}",
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van de Wiel-van Kemenade, E, van Kooyk, Y, de Boer, AJ, Huijbens, RJ, Weder, P, van de Kasteele, W, Melief, CJ & Figdor, CG 1992, 'Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA' Journal of Cell Biology, vol. 117, no. 2, pp. 461-70.

Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA. / van de Wiel-van Kemenade, E; van Kooyk, Y; de Boer, A J; Huijbens, R J; Weder, P; van de Kasteele, W; Melief, C J; Figdor, C G.

In: Journal of Cell Biology, Vol. 117, No. 2, 04.1992, p. 461-70.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA

AU - van de Wiel-van Kemenade, E

AU - van Kooyk, Y

AU - de Boer, A J

AU - Huijbens, R J

AU - Weder, P

AU - van de Kasteele, W

AU - Melief, C J

AU - Figdor, C G

PY - 1992/4

Y1 - 1992/4

N2 - Investigating the regulation of very late antigen (VLA)-mediated functions, we found that TS2/16, a mAb directed against the beta chain of the VLA group of integrins, can induce binding of resting peripheral blood lymphocytes, cloned T lymphocytes, and Epstein Barr virus-transformed B cells to extracellular matrix components, fibronectin, laminin, and collagen, but not to fibrinogen. The antibody stimulates VLA-4-, VLA-5-, and VLA-6-mediated binding. Furthermore, it induces VLA-4-mediated binding to vascular cell adhesion molecule-1 expressed by rTNF-alpha-stimulated endothelial cells, but it does not stimulate homotypic aggregation of cells as described for a number of anti-VLA-4 alpha antibodies (Bednarczyk, J.L., and B. W. McIntyre. 1990. J. Immunol. 144: 777-784; Campanero, M. R., R. Pulido, M. A. Ursa, M. Rodríguez-Moya, M. O. de Landázuri, and F. Sánchez-Madrid. 1990. J. Cell Biol. 110:2157-2165). Therefore, the stimulating activity of this anti-beta 1 antibody clearly contrasts with that of the anti-VLA-4 alpha antibodies, which induce homotypic cell aggregation, but not binding of cells to extracellular matrix components or endothelial cells, indicating that TS2/16 may generate different signals. The observation that also F(ab')2 or Fab fragments of this anti-beta 1 antibody stimulate binding to extracellular matrix components and endothelial cells excludes the possibility that binding requires receptor crosslinking, or is Fc receptor mediated. Induction of this adhesion is cation and energy dependent and requires an intact cytoskeleton. Although changes in the conformation of VLA integrins induced by this antibody may regulate their functional activity, the dependence on metabolic energy indicates that intracellular processes may also play a role.

AB - Investigating the regulation of very late antigen (VLA)-mediated functions, we found that TS2/16, a mAb directed against the beta chain of the VLA group of integrins, can induce binding of resting peripheral blood lymphocytes, cloned T lymphocytes, and Epstein Barr virus-transformed B cells to extracellular matrix components, fibronectin, laminin, and collagen, but not to fibrinogen. The antibody stimulates VLA-4-, VLA-5-, and VLA-6-mediated binding. Furthermore, it induces VLA-4-mediated binding to vascular cell adhesion molecule-1 expressed by rTNF-alpha-stimulated endothelial cells, but it does not stimulate homotypic aggregation of cells as described for a number of anti-VLA-4 alpha antibodies (Bednarczyk, J.L., and B. W. McIntyre. 1990. J. Immunol. 144: 777-784; Campanero, M. R., R. Pulido, M. A. Ursa, M. Rodríguez-Moya, M. O. de Landázuri, and F. Sánchez-Madrid. 1990. J. Cell Biol. 110:2157-2165). Therefore, the stimulating activity of this anti-beta 1 antibody clearly contrasts with that of the anti-VLA-4 alpha antibodies, which induce homotypic cell aggregation, but not binding of cells to extracellular matrix components or endothelial cells, indicating that TS2/16 may generate different signals. The observation that also F(ab')2 or Fab fragments of this anti-beta 1 antibody stimulate binding to extracellular matrix components and endothelial cells excludes the possibility that binding requires receptor crosslinking, or is Fc receptor mediated. Induction of this adhesion is cation and energy dependent and requires an intact cytoskeleton. Although changes in the conformation of VLA integrins induced by this antibody may regulate their functional activity, the dependence on metabolic energy indicates that intracellular processes may also play a role.

KW - Antibodies, Monoclonal/immunology

KW - B-Lymphocytes/physiology

KW - Cell Adhesion

KW - Cell Aggregation

KW - Cell Line

KW - Cell Line, Transformed

KW - Cells, Cultured

KW - Collagen/metabolism

KW - Endothelium/physiology

KW - Extracellular Matrix/physiology

KW - Fibrinogen/metabolism

KW - Fibronectins/metabolism

KW - Humans

KW - Laminin/metabolism

KW - Receptors, Very Late Antigen/immunology

KW - T-Lymphocytes/physiology

KW - Tetradecanoylphorbol Acetate/pharmacology

M3 - Article

VL - 117

SP - 461

EP - 470

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

ER -

van de Wiel-van Kemenade E, van Kooyk Y, de Boer AJ, Huijbens RJ, Weder P, van de Kasteele W et al. Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA. Journal of Cell Biology. 1992 Apr;117(2):461-70.