Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18

R.D. Fontijn, J. Favre, B.A. Naaijkens, E. Meinster, N.J. Paauw, S.L. Ragghoe, T.D. Nauta, M.A. van den Broek, E.M. Weijers, H.W.M. Niessen, P. Koolwijk, A.J.G. Horrevoets

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.

Original languageEnglish
Pages (from-to)367-378
Number of pages12
JournalStem Cell Research
Volume13
Issue number3 Pt A
DOIs
Publication statusPublished - Nov 2014

Cite this

Fontijn, R.D. ; Favre, J. ; Naaijkens, B.A. ; Meinster, E. ; Paauw, N.J. ; Ragghoe, S.L. ; Nauta, T.D. ; van den Broek, M.A. ; Weijers, E.M. ; Niessen, H.W.M. ; Koolwijk, P. ; Horrevoets, A.J.G. / Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18. In: Stem Cell Research. 2014 ; Vol. 13, No. 3 Pt A. pp. 367-378.
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title = "Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18",
abstract = "Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.",
keywords = "Adipose Tissue, Cell Differentiation, Cell Movement, Cells, Cultured, Cellular Reprogramming, Chemotaxis, Endothelial Cells, Genetic Vectors, Human Umbilical Vein Endothelial Cells, Humans, Matrix Metalloproteinase 7, Microtubules, SOXF Transcription Factors, Shear Strength, Stromal Cells, Vascular Endothelial Growth Factor A, Journal Article, Research Support, Non-U.S. Gov't",
author = "R.D. Fontijn and J. Favre and B.A. Naaijkens and E. Meinster and N.J. Paauw and S.L. Ragghoe and T.D. Nauta and {van den Broek}, M.A. and E.M. Weijers and H.W.M. Niessen and P. Koolwijk and A.J.G. Horrevoets",
note = "Copyright {\circledC} 2014. Published by Elsevier B.V.",
year = "2014",
month = "11",
doi = "10.1016/j.scr.2014.09.004",
language = "English",
volume = "13",
pages = "367--378",
journal = "Stem Cell Research",
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Fontijn, RD, Favre, J, Naaijkens, BA, Meinster, E, Paauw, NJ, Ragghoe, SL, Nauta, TD, van den Broek, MA, Weijers, EM, Niessen, HWM, Koolwijk, P & Horrevoets, AJG 2014, 'Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18' Stem Cell Research, vol. 13, no. 3 Pt A, pp. 367-378. https://doi.org/10.1016/j.scr.2014.09.004

Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18. / Fontijn, R.D.; Favre, J.; Naaijkens, B.A.; Meinster, E.; Paauw, N.J.; Ragghoe, S.L.; Nauta, T.D.; van den Broek, M.A.; Weijers, E.M.; Niessen, H.W.M.; Koolwijk, P.; Horrevoets, A.J.G.

In: Stem Cell Research, Vol. 13, No. 3 Pt A, 11.2014, p. 367-378.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18

AU - Fontijn, R.D.

AU - Favre, J.

AU - Naaijkens, B.A.

AU - Meinster, E.

AU - Paauw, N.J.

AU - Ragghoe, S.L.

AU - Nauta, T.D.

AU - van den Broek, M.A.

AU - Weijers, E.M.

AU - Niessen, H.W.M.

AU - Koolwijk, P.

AU - Horrevoets, A.J.G.

N1 - Copyright © 2014. Published by Elsevier B.V.

PY - 2014/11

Y1 - 2014/11

N2 - Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.

AB - Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.

KW - Adipose Tissue

KW - Cell Differentiation

KW - Cell Movement

KW - Cells, Cultured

KW - Cellular Reprogramming

KW - Chemotaxis

KW - Endothelial Cells

KW - Genetic Vectors

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - Matrix Metalloproteinase 7

KW - Microtubules

KW - SOXF Transcription Factors

KW - Shear Strength

KW - Stromal Cells

KW - Vascular Endothelial Growth Factor A

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.scr.2014.09.004

DO - 10.1016/j.scr.2014.09.004

M3 - Article

VL - 13

SP - 367

EP - 378

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 3 Pt A

ER -