An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells

Katharina Herzog, Lodewijk Ijlst, Arno G. van Cruchten, Carlo W. T. van Roermund, Wim Kulik, Ronald J. A. Wanders, Hans R. Waterham

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).
Original languageEnglish
Article number45
JournalMetabolites
Volume9
Issue number3
DOIs
Publication statusPublished - 1 Mar 2019
Externally publishedYes

Cite this

Herzog, Katharina ; Ijlst, Lodewijk ; van Cruchten, Arno G. ; van Roermund, Carlo W. T. ; Kulik, Wim ; Wanders, Ronald J. A. ; Waterham, Hans R. / An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells. In: Metabolites. 2019 ; Vol. 9, No. 3.
@article{e1d5ce90456a4ec385b10f9d6924ecae,
title = "An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells",
abstract = "Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).",
author = "Katharina Herzog and Lodewijk Ijlst and {van Cruchten}, {Arno G.} and {van Roermund}, {Carlo W. T.} and Wim Kulik and Wanders, {Ronald J. A.} and Waterham, {Hans R.}",
year = "2019",
month = "3",
day = "1",
doi = "10.3390/metabo9030045",
language = "English",
volume = "9",
journal = "Metabolites",
issn = "2218-1989",
number = "3",

}

An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells. / Herzog, Katharina; Ijlst, Lodewijk; van Cruchten, Arno G.; van Roermund, Carlo W. T.; Kulik, Wim; Wanders, Ronald J. A.; Waterham, Hans R.

In: Metabolites, Vol. 9, No. 3, 45, 01.03.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells

AU - Herzog, Katharina

AU - Ijlst, Lodewijk

AU - van Cruchten, Arno G.

AU - van Roermund, Carlo W. T.

AU - Kulik, Wim

AU - Wanders, Ronald J. A.

AU - Waterham, Hans R.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).

AB - Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064521253&origin=inward

U2 - 10.3390/metabo9030045

DO - 10.3390/metabo9030045

M3 - Article

VL - 9

JO - Metabolites

JF - Metabolites

SN - 2218-1989

IS - 3

M1 - 45

ER -