Ankyrin repeat and zinc-finger domain-containing 1 mutations are associated with infantile-onset inflammatory bowel disease

Désirée Y. Van Haaften-Visser, Magdalena Harakalova, Enric Mocholi, Joris M. Van Montfrans, Abdul Elkadri, Ester Rieter, Karoline Fiedler, Peter M. Van Hasselt, Emily M.M. Triffaux, Mieke M. Van Haelst, Isaac J. Nijman, Wigard P. Kloosterman, Edward E.S. Nieuwenhuis, Aleixo M. Muise, Edwin Cuppen, Roderick H.J. Houwen, Paul J. Coffer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Infantile-onset inflammatory bowel disease (IO IBD) is an invalidating illness with an onset before 2 years of age and has a complex pathophysiology in which genetic factors are important. Homozygosity mapping and whole-exome sequencing in an IO IBD patient and subsequent sequencing of the candidate gene in 12 additional IO IBD patients revealed two patients with two mutated ankyrin repeat and zinc-finger domain-containing 1 (ANKZF1) alleles (homozygous ANKZF1 R585Q mutation and compound heterozygous ANKZF1 E152K and V32-Q87del mutations, respectively) and two patients with one mutated ANKZF1 allele. Although the function of ANKZF1 in mammals had not been previously evaluated, we show that ANKZF1 has an indispensable role in the mitochondrial response to cellular stress. ANKZF1 is located diffusely in the cytoplasm and translocates to the mitochondria upon cellular stress. ANKZF1 depletion reduces mitochondrial integrity and mitochondrial respiration under conditions of cellular stress. The ANKZF1 mutations identified in IO IBD patients with two mutated ANKZF1 alleles result in dysfunctional ANKZF1, as shown by an increased level of apoptosis in patients' lymphocytes, a decrease in mitochondrial respiration in patient fibroblasts with a homozygous ANKZF1 R585Q mutation, and an inability of ANKZF1 R585Q and E152K to rescue the phenotype of yeast deficient in Vms1, the yeast homologue of ANKZF1. These data indicate that loss-of-function mutations in ANKZF1 result in deregulation of mitochondrial integrity, and this may play a pathogenic role in the development of IO IBD.

Original languageEnglish
Pages (from-to)7904-7920
Number of pages17
JournalJournal of Biological Chemistry
Volume292
Issue number19
DOIs
Publication statusPublished - 12 May 2017

Cite this

Van Haaften-Visser, D. Y., Harakalova, M., Mocholi, E., Van Montfrans, J. M., Elkadri, A., Rieter, E., Fiedler, K., Van Hasselt, P. M., Triffaux, E. M. M., Van Haelst, M. M., Nijman, I. J., Kloosterman, W. P., Nieuwenhuis, E. E. S., Muise, A. M., Cuppen, E., Houwen, R. H. J., & Coffer, P. J. (2017). Ankyrin repeat and zinc-finger domain-containing 1 mutations are associated with infantile-onset inflammatory bowel disease. Journal of Biological Chemistry, 292(19), 7904-7920. https://doi.org/10.1074/jbc.M116.772038