Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats: a pathway for the induction of non-specific symptoms of sickness?

Anne Marie van Dam, Madeleine Brouns, Simone Louisse, Frank Berkenbosch*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


The presence and cellular localization of interleukin-1β immunoreactivity (irIL-1) in and around the brain was investigated using immunocytochemistry on Bouin's fixed vibratome brain sections of control and endotoxin-treated rats. Peripheral administration of endotoxin resulted in the appearence of irIL-1 in cells in the meninges, choroid plexus, brain blood vessels and in non-neuronal cells in the brain parenchyma. Using monoclonal and polyclonal antibodies to macrophage and astrocyte antigens, the endotoxin-induced irIL-1 positive cells could be identified as macrophages in the meninges and choroid plexus (ED2), perivascular cells (ED2) and ramified microglial cells (GSA-I-B4 isolectin). Our data demonstrate a pathway for the induction of non-specific sickness symptoms in response to endotoxin.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalBrain Research
Issue number2
Publication statusPublished - 21 Aug 1992

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