Applying CRISPR-Cas9 Genome Editing to Study Genes Involved in Peroxisome Biogenesis or Peroxisomal Functions

Serhii Chornyi, Janet Koster, Hans R. Waterham

Research output: Contribution to journalArticleAcademicpeer-review


The development and application of the CRISPR-Cas9 technology for genome editing of mammalian cells have opened up a wealth of possibilities for genetically modifying and manipulating human cells, and use in functional studies or therapeutic approaches.Here we describe the approach that we have been using successfully to generate multiple human cell lines with targeted (partial) gene deletions, i.e., knockout cells, or human cells with modified genomic nucleotide sequences, i.e., knock-in cells, in genes encoding known or putative proteins involved in peroxisome biogenesis or peroxisomal functions.
Original languageEnglish
Pages (from-to)233-245
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 2023
Externally publishedYes

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