Assembly of microarrays for genome-wide measurement of DNA copy number

Antoine M. Snijders, Norma Nowak, Richard Segraves, Stephanie Blackwood, Nils Brown, Jeffrey Conroy, Greg Hamilton, Anna Katherine Hindle, Bing Huey, Karen Kimura, Sindy Law, Ken Myambo, Joel Palmer, Bauke Ylstra, Jingzhu Pearl Yue, Joe W. Gray, Ajay N. Jain, Daniel Pinkel, Donna G. Albertson*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

Original languageEnglish
Pages (from-to)263-264
Number of pages2
JournalNature Genetics
Volume29
Issue number3
DOIs
Publication statusPublished - 10 Dec 2001

Cite this

Snijders, A. M., Nowak, N., Segraves, R., Blackwood, S., Brown, N., Conroy, J., ... Albertson, D. G. (2001). Assembly of microarrays for genome-wide measurement of DNA copy number. Nature Genetics, 29(3), 263-264. https://doi.org/10.1038/ng754