Association between HIV-1 subtype and drug resistance in Nigerian infants

Beth Chaplin, Alani Sulaimon Akanmu, Seth C Inzaule, Jay Osi Samuels, Prosper Okonkwo, Oluwafunke Ilesanmi, Isaac F A Adewole, Chukwuemeka Asadu, Hadiza Khamofu, Rex Mpazanje, Nicaise Ndembi, Solomon Odafe, Kim C E Sigaloff, Evelyn Nkadi Ngige, Emmanuel Okechukwu Abatta, Gbenga Akinbiyi, Patrick Dakum, Tobias F Rinke de Wit, Phyllis Kanki

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: Many lines of evidence point to HIV-1 subtype-specific differences in the development of drug resistance mutations. While variation between subtype C and others has been extensively explored, there has been less emphasis on subtypes common to West Africa. We examined a previously described national survey of pretreatment drug resistance in HIV-1-infected Nigerian children aged <18 months, to explore the association between subtypes and patterns of resistance.

Methods: Five hundred and forty-nine dried blood spots, from 15 early infant diagnostic facilities in Nigeria, were amplified and HIV-1 polymerase was sequenced. Four hundred and twenty-four were analysed for surveillance drug resistance mutations (SDRMs). Associations between subtype and SDRMs were evaluated by Fisher's exact test and logistic regression analysis, controlling for geographical region and exposure.

Results: Using the sub-subtypes of HIV-1 G defined by Delatorre et al. (PLoS One 2014; 9: e98908) the most common subtypes were CRF02_AG (174, 41.0%), GWA-I (128, 30.2%), GWA-II (24, 5.7%), GCA (11, 2.6%), A (21, 5.0%) and CRF06_cpx (18, 4.2%). One hundred and ninety infants (44.8%) had ≥1 NNRTI mutation, 92 infants (21.7%) had ≥1 NRTI mutation and 6 infants (1.4%) had ≥1 PI mutation. By logistic regression, 67N was more common in GWA-II/GCA than CRF02_AG (OR 12.0, P = 0.006), as was 70R (OR 23.1, P = 0.007), 184I/V (OR 2.92, P = 0.020), the presence of ≥1 thymidine analogue mutation (TAM) (OR 3.87, P = 0.014), ≥1 type 2 TAM (OR 7.61, P = 0.001) and ≥1 NRTI mutation (OR 3.26, P = 0.005).

Conclusions: This dataset reveals differences among SDRMs by subtype; in particular, between the GWA-II and GCA subclades, compared with CRF02_AG and GWA-I.

Original languageEnglish
Pages (from-to)172-176
JournalJournal of Antimicrobial Chemotherapy
Volume74
Issue number1
Early online date22 Sep 2018
DOIs
Publication statusPublished - Jan 2019

Cite this

Chaplin, B., Akanmu, A. S., Inzaule, S. C., Samuels, J. O., Okonkwo, P., Ilesanmi, O., ... Kanki, P. (2019). Association between HIV-1 subtype and drug resistance in Nigerian infants. Journal of Antimicrobial Chemotherapy, 74(1), 172-176. https://doi.org/10.1093/jac/dky380