Objectives: To investigate the potential association between beta blocker use and hearing ability in adults and to discern whether this effect is dose-dependent. Design: Cross-sectional analyses. Multiple linear regression was performed with hearing ability as the dependent variable and beta blocker use as the independent variable. The independent variable was classified into three dose categories for secondary analysis. Adjustments were made for age, gender, educational level, and tobacco smoking status. Study sample: 1636 adults, 75 of whom reported being on beta blockers, from the internet-based Netherlands Longitudinal Study on Hearing (NL-SH). Results: No significant association was found between beta blocker use and hearing ability in noise. In the adjusted regressions, beta blocker use changed the speech reception threshold in noise (SRT) by −0.04 dB signal-to-noise ratio (SNR) (95%CI [−0.67 to 0.58], p = 0.890). Medium dose beta blocker use changed SRT by −0.42 dB SNR (95%CI [−1.38 to 0.71], p = 0.433), while a high dose changed it by −0.26 dB SNR (95%CI [−1.74 to 1.4], p = 0.767). Conclusions: No evidence was found for beta blocker-induced changes in hearing ability. Future studies on this topic should favour case-control and cohort study designs, while focussing on a hypertensive population to minimise confounding by indication.