Augmentation of antigen-specific lymphoproliferative responses in vitro by biological response modifiers

T. D. De Gruijl, J. J. Moore, E. De Vries, B. M.E. Von Blomberg-Van der Flier, J. C.M. Fonk, R. J. Scheper*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


The detection of antigen-specific T cell responsiveness, particularly of resting memory lymphocytes, in cultures of peripheral blood mononuclear cells (PBMC) may be hampered by a less than optimal antigen presentation in vitro. Augmented sensitivity of the test system may be achieved by the addition of reagents with a beneficial effect on lymphocyte and antigen-presenting cell (APC) functions. In this study the effect of several biological response modifiers on antigen-specific T cell proliferation was determined, using nickel sulphate and tetanus toxoid as test antigens. IL-1α (100 U/ml), interferon-gamma (IFN-γ) (10 U/ml), and indomethacin (2 μM) were found to significantly enhance nickel-induced proliferation in PBMC cultures from nickel-hypersensitive donors (n = 6). Tetanus-induced proliferation (n = 5) was similarly enhanced, both by the above supplements and by the addition of polyethylene glycol (PEG) or a neuraminidase treatment of the PBMC before culture. The addition to PBMC cultures of a combination of IL-1α (30 U/ml), IFN-γ (10 U/ml), and indomethacin (2 μM) is recommended to specifically enhance antigen-induced lymphoproliferative signals.

Original languageEnglish
Pages (from-to)535-540
Number of pages6
JournalClinical and Experimental Immunology
Issue number3
Publication statusPublished - 1 Jan 1994

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