BAC to the future! Or oligonucleotides: A perspective for micro array comparative genomic hybridization (array CGH)

Bauke Ylstra*, Paul van den IJssel, Beatriz Carvalho, Ruud H. Brakenhoff, Gerrit A. Meijer

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review


The array CGH technique (Array Comparative Genome Hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. It is used in human genetics and oncology, with great promise for clinical application. Until recently primarily PCR amplified bacterial artificial chromosomes (BACs) or cDNAs have been spotted as elements on the array. The large-scale DNA isolations or PCR amplifications of the large-insert clones necessary for manufacturing the arrays are elaborate and time-consuming. Lack of a high-resolution highly sensitive (commercial) alternative has undoubtedly hindered the implementation of array CGH in research and diagnostics. Recently, synthetic oligonucleotides as arrayed elements have been introduced as an alternative substrate for array CGH, both by academic institutions as well as by commercial providers. Oligonucleotide libraries or ready-made arrays can be bought off-the-shelf saving considerable time and efforts. For RNA expression profiling, we have seen a gradual transition from in-house printed cDNA-based expression arrays to oligonucleotide arrays and we expect a similar transition for array CGH. This review compares the different platforms and will attempt to shine a light on the 'BAC to the future' of the array CGH technique.

Original languageEnglish
Pages (from-to)445-450
Number of pages6
JournalNucleic Acids Research
Issue number2
Publication statusPublished - 1 Feb 2006

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