Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Rhesi Kristiana, Gilles Bedoux, Gerard Pals, I. Wayan Mudianta, Laure Taupin, Christel Marty, Meezan Ardhanu Asagabaldan, Diah Ayuningrum, Agus Trianto, Nathalie Bourgougnon, Ocky Karna Radjasa, Agus Sabdono, Muhammad Hanafi

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant.
Original languageEnglish
Article numbere8093
JournalPeerJ
Volume2020
Issue number1
DOIs
Publication statusPublished - 2020

Cite this

Kristiana, R., Bedoux, G., Pals, G., Wayan Mudianta, I., Taupin, L., Marty, C., ... Hanafi, M. (2020). Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia. PeerJ, 2020(1), [e8093]. https://doi.org/10.7717/peerj.8093
Kristiana, Rhesi ; Bedoux, Gilles ; Pals, Gerard ; Wayan Mudianta, I. ; Taupin, Laure ; Marty, Christel ; Asagabaldan, Meezan Ardhanu ; Ayuningrum, Diah ; Trianto, Agus ; Bourgougnon, Nathalie ; Radjasa, Ocky Karna ; Sabdono, Agus ; Hanafi, Muhammad. / Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia. In: PeerJ. 2020 ; Vol. 2020, No. 1.
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title = "Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia",
abstract = "The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant.",
author = "Rhesi Kristiana and Gilles Bedoux and Gerard Pals and {Wayan Mudianta}, I. and Laure Taupin and Christel Marty and Asagabaldan, {Meezan Ardhanu} and Diah Ayuningrum and Agus Trianto and Nathalie Bourgougnon and Radjasa, {Ocky Karna} and Agus Sabdono and Muhammad Hanafi",
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Kristiana, R, Bedoux, G, Pals, G, Wayan Mudianta, I, Taupin, L, Marty, C, Asagabaldan, MA, Ayuningrum, D, Trianto, A, Bourgougnon, N, Radjasa, OK, Sabdono, A & Hanafi, M 2020, 'Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia' PeerJ, vol. 2020, no. 1, e8093. https://doi.org/10.7717/peerj.8093

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia. / Kristiana, Rhesi; Bedoux, Gilles; Pals, Gerard; Wayan Mudianta, I.; Taupin, Laure; Marty, Christel; Asagabaldan, Meezan Ardhanu; Ayuningrum, Diah; Trianto, Agus; Bourgougnon, Nathalie; Radjasa, Ocky Karna; Sabdono, Agus; Hanafi, Muhammad.

In: PeerJ, Vol. 2020, No. 1, e8093, 2020.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

AU - Kristiana, Rhesi

AU - Bedoux, Gilles

AU - Pals, Gerard

AU - Wayan Mudianta, I.

AU - Taupin, Laure

AU - Marty, Christel

AU - Asagabaldan, Meezan Ardhanu

AU - Ayuningrum, Diah

AU - Trianto, Agus

AU - Bourgougnon, Nathalie

AU - Radjasa, Ocky Karna

AU - Sabdono, Agus

AU - Hanafi, Muhammad

PY - 2020

Y1 - 2020

N2 - The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant.

AB - The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant.

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Kristiana R, Bedoux G, Pals G, Wayan Mudianta I, Taupin L, Marty C et al. Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia. PeerJ. 2020;2020(1). e8093. https://doi.org/10.7717/peerj.8093