Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study

Helena Ukkonen, Simo Vuokila, Jopi J W Mikkonen, Hannah Dekker, Engelbert A J M Schulten, Elisabeth Bloemena, Arto Koistinen, Tulio A Valdez, Arja M Kullaa, Surya Pratap Singh

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Spectra extracted from this layer suggests changes in overall nucleic acid and protein content in response to the therapeutic irradiation. Discrimination among control and irradiated groups have been achieved using principal component analysis. Findings of this preliminary study further support prospective utilization of Fourier Transform InfraRed (FTIR) imaging as a non-destructive, label-free tool for objective assessment of the oral mucosa in patient groups with or without radiation therapy.

LanguageEnglish
JournalBiosensors
Volume9
Issue number1
DOIs
Publication statusPublished - 13 Jan 2019

Cite this

Ukkonen, Helena ; Vuokila, Simo ; Mikkonen, Jopi J W ; Dekker, Hannah ; Schulten, Engelbert A J M ; Bloemena, Elisabeth ; Koistinen, Arto ; Valdez, Tulio A ; Kullaa, Arja M ; Singh, Surya Pratap. / Biochemical Changes in Irradiated Oral Mucosa : A FTIR Spectroscopic Study. In: Biosensors. 2019 ; Vol. 9, No. 1.
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title = "Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study",
abstract = "Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Spectra extracted from this layer suggests changes in overall nucleic acid and protein content in response to the therapeutic irradiation. Discrimination among control and irradiated groups have been achieved using principal component analysis. Findings of this preliminary study further support prospective utilization of Fourier Transform InfraRed (FTIR) imaging as a non-destructive, label-free tool for objective assessment of the oral mucosa in patient groups with or without radiation therapy.",
author = "Helena Ukkonen and Simo Vuokila and Mikkonen, {Jopi J W} and Hannah Dekker and Schulten, {Engelbert A J M} and Elisabeth Bloemena and Arto Koistinen and Valdez, {Tulio A} and Kullaa, {Arja M} and Singh, {Surya Pratap}",
year = "2019",
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doi = "10.3390/bios9010012",
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Ukkonen, H, Vuokila, S, Mikkonen, JJW, Dekker, H, Schulten, EAJM, Bloemena, E, Koistinen, A, Valdez, TA, Kullaa, AM & Singh, SP 2019, 'Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study', Biosensors, vol. 9, no. 1. https://doi.org/10.3390/bios9010012

Biochemical Changes in Irradiated Oral Mucosa : A FTIR Spectroscopic Study. / Ukkonen, Helena; Vuokila, Simo; Mikkonen, Jopi J W; Dekker, Hannah; Schulten, Engelbert A J M; Bloemena, Elisabeth; Koistinen, Arto; Valdez, Tulio A; Kullaa, Arja M; Singh, Surya Pratap.

In: Biosensors, Vol. 9, No. 1, 13.01.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Biochemical Changes in Irradiated Oral Mucosa

T2 - Biosensors

AU - Ukkonen, Helena

AU - Vuokila, Simo

AU - Mikkonen, Jopi J W

AU - Dekker, Hannah

AU - Schulten, Engelbert A J M

AU - Bloemena, Elisabeth

AU - Koistinen, Arto

AU - Valdez, Tulio A

AU - Kullaa, Arja M

AU - Singh, Surya Pratap

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Y1 - 2019/1/13

N2 - Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Spectra extracted from this layer suggests changes in overall nucleic acid and protein content in response to the therapeutic irradiation. Discrimination among control and irradiated groups have been achieved using principal component analysis. Findings of this preliminary study further support prospective utilization of Fourier Transform InfraRed (FTIR) imaging as a non-destructive, label-free tool for objective assessment of the oral mucosa in patient groups with or without radiation therapy.

AB - Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Spectra extracted from this layer suggests changes in overall nucleic acid and protein content in response to the therapeutic irradiation. Discrimination among control and irradiated groups have been achieved using principal component analysis. Findings of this preliminary study further support prospective utilization of Fourier Transform InfraRed (FTIR) imaging as a non-destructive, label-free tool for objective assessment of the oral mucosa in patient groups with or without radiation therapy.

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JO - Biosensors

JF - Biosensors

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