Cancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles

Sander C. Steenbeek, Thang V. Pham, Joep de Ligt, Anoek Zomer, Jaco C. Knol, Sander R. Piersma, Tim Schelfhorst, Rick Huisjes, Raymond M. Schiffelers, Edwin Cuppen, Connie R. Jimenez, Jacco van Rheenen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Recent data showed that cancer cells from different tumor subtypes with distinct metastatic potential influence each other's metastatic behavior by exchanging biomolecules through extracellular vesicles (EVs). However, it is debated how small amounts of cargo can mediate this effect, especially in tumors where all cells are from one subtype, and only subtle molecular differences drive metastatic heterogeneity. To study this, we have characterized the content of EVs shed in vivo by two clones of melanoma (B16) tumors with distinct metastatic potential. Using the Cre-LoxP system and intravital microscopy, we show that cells from these distinct clones phenocopy their migratory behavior through EV exchange. By tandem mass spectrometry and RNA sequencing, we show that EVs shed by these clones into the tumor microenvironment contain thousands of different proteins and RNAs, and many of these biomolecules are from interconnected signaling networks involved in cellular processes such as migration. Thus, EVs contain numerous proteins and RNAs and act on recipient cells by invoking a multi-faceted biological response including cell migration.
Original languageEnglish
Article numbere98357
JournalEMBO Journal
Volume37
Issue number15
DOIs
Publication statusPublished - 2018

Cite this

Steenbeek, Sander C. ; Pham, Thang V. ; de Ligt, Joep ; Zomer, Anoek ; Knol, Jaco C. ; Piersma, Sander R. ; Schelfhorst, Tim ; Huisjes, Rick ; Schiffelers, Raymond M. ; Cuppen, Edwin ; Jimenez, Connie R. ; van Rheenen, Jacco. / Cancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles. In: EMBO Journal. 2018 ; Vol. 37, No. 15.
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title = "Cancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles",
abstract = "Recent data showed that cancer cells from different tumor subtypes with distinct metastatic potential influence each other's metastatic behavior by exchanging biomolecules through extracellular vesicles (EVs). However, it is debated how small amounts of cargo can mediate this effect, especially in tumors where all cells are from one subtype, and only subtle molecular differences drive metastatic heterogeneity. To study this, we have characterized the content of EVs shed in vivo by two clones of melanoma (B16) tumors with distinct metastatic potential. Using the Cre-LoxP system and intravital microscopy, we show that cells from these distinct clones phenocopy their migratory behavior through EV exchange. By tandem mass spectrometry and RNA sequencing, we show that EVs shed by these clones into the tumor microenvironment contain thousands of different proteins and RNAs, and many of these biomolecules are from interconnected signaling networks involved in cellular processes such as migration. Thus, EVs contain numerous proteins and RNAs and act on recipient cells by invoking a multi-faceted biological response including cell migration.",
author = "Steenbeek, {Sander C.} and Pham, {Thang V.} and {de Ligt}, Joep and Anoek Zomer and Knol, {Jaco C.} and Piersma, {Sander R.} and Tim Schelfhorst and Rick Huisjes and Schiffelers, {Raymond M.} and Edwin Cuppen and Jimenez, {Connie R.} and {van Rheenen}, Jacco",
year = "2018",
doi = "10.15252/embj.201798357",
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Steenbeek, SC, Pham, TV, de Ligt, J, Zomer, A, Knol, JC, Piersma, SR, Schelfhorst, T, Huisjes, R, Schiffelers, RM, Cuppen, E, Jimenez, CR & van Rheenen, J 2018, 'Cancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles' EMBO Journal, vol. 37, no. 15, e98357. https://doi.org/10.15252/embj.201798357

Cancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles. / Steenbeek, Sander C.; Pham, Thang V.; de Ligt, Joep; Zomer, Anoek; Knol, Jaco C.; Piersma, Sander R.; Schelfhorst, Tim; Huisjes, Rick; Schiffelers, Raymond M.; Cuppen, Edwin; Jimenez, Connie R.; van Rheenen, Jacco.

In: EMBO Journal, Vol. 37, No. 15, e98357, 2018.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Steenbeek, Sander C.

AU - Pham, Thang V.

AU - de Ligt, Joep

AU - Zomer, Anoek

AU - Knol, Jaco C.

AU - Piersma, Sander R.

AU - Schelfhorst, Tim

AU - Huisjes, Rick

AU - Schiffelers, Raymond M.

AU - Cuppen, Edwin

AU - Jimenez, Connie R.

AU - van Rheenen, Jacco

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