CD31-positive microvessel density within adenomas of Lynch Syndrome patients is similar compared to adenomas of non-Lynch patients

Jasper L A Vleugels, Sanne M van Neerven, Monique E van Leerdam, Linda K Wanders, Meike de Wit, Beatriz Carvalho, Pien M Delis-van Diemen, Frank G J Kallenberg, Louis Vermeulen, Jeroen A Beliën, James E East, Gerrit A Meijer, Evelien Dekker

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background and study aims  Microsatellite instability accelerates colorectal cancer development in patients with Lynch syndrome (LS). Previous research showed that virtual chromoendoscopy increases detection of adenomas during colonoscopy surveillance of patients with LS. Because previous research revealed that Lynch patients have an increased vascular network in the oral mucosa, we hypothesized that increased vascularization of LS-associated adenomas is the cause of better detection with virtual chromoendoscopy. Patients and methods  In this pilot study, patients with LS having a proven germline mutation were selected from two tertiary referral hospitals and non-LS patients from an outpatient colonoscopy center. Adenomas from patients with LS were exactly matched in size and histology with adenomas from non-LS patients. Initial adenoma diagnosis was confirmed by a specialist pathologist. All adenomas were stained with CD31 and adenomatous tissue was annotated by the specialist pathologist. Image analysis of CD31-positive microvessel density was conducted using FIJI software. Results  Colonoscopy of 63 patients with LS and 24 non-LS patients provided 40 adenomas that could be exactly matched in size and histology. In image-analysis, the CD31-positive microvessel density (2.49 % vs. 2.47 %, P  = 0.96), the average size of CD31-positive structures (514 μm 2 vs. 523 μm 2 , P  = 0.26) nor the amount of vascular structures per mm 2 (183 vs. 176, P  = 0.50) differed between adenomas of LS patients and non-Lynch patients. Conclusion  The outcomes of this pilot case-control study did not provide further insights into the mechanism of increased adenoma detection in LS patients using virtual chromoendoscopy techniques.

Original languageEnglish
Pages (from-to)E701-E707
JournalEndoscopy International Open
Volume7
Issue number5
DOIs
Publication statusPublished - May 2019

Cite this

Vleugels, J. L. A., van Neerven, S. M., van Leerdam, M. E., Wanders, L. K., de Wit, M., Carvalho, B., ... Dekker, E. (2019). CD31-positive microvessel density within adenomas of Lynch Syndrome patients is similar compared to adenomas of non-Lynch patients. Endoscopy International Open, 7(5), E701-E707. https://doi.org/10.1055/a-0832-8283
Vleugels, Jasper L A ; van Neerven, Sanne M ; van Leerdam, Monique E ; Wanders, Linda K ; de Wit, Meike ; Carvalho, Beatriz ; Delis-van Diemen, Pien M ; Kallenberg, Frank G J ; Vermeulen, Louis ; Beliën, Jeroen A ; East, James E ; Meijer, Gerrit A ; Dekker, Evelien. / CD31-positive microvessel density within adenomas of Lynch Syndrome patients is similar compared to adenomas of non-Lynch patients. In: Endoscopy International Open. 2019 ; Vol. 7, No. 5. pp. E701-E707.
@article{363fabe781b342598758a4322be8d1ce,
title = "CD31-positive microvessel density within adenomas of Lynch Syndrome patients is similar compared to adenomas of non-Lynch patients",
abstract = "Background and study aims  Microsatellite instability accelerates colorectal cancer development in patients with Lynch syndrome (LS). Previous research showed that virtual chromoendoscopy increases detection of adenomas during colonoscopy surveillance of patients with LS. Because previous research revealed that Lynch patients have an increased vascular network in the oral mucosa, we hypothesized that increased vascularization of LS-associated adenomas is the cause of better detection with virtual chromoendoscopy. Patients and methods  In this pilot study, patients with LS having a proven germline mutation were selected from two tertiary referral hospitals and non-LS patients from an outpatient colonoscopy center. Adenomas from patients with LS were exactly matched in size and histology with adenomas from non-LS patients. Initial adenoma diagnosis was confirmed by a specialist pathologist. All adenomas were stained with CD31 and adenomatous tissue was annotated by the specialist pathologist. Image analysis of CD31-positive microvessel density was conducted using FIJI software. Results  Colonoscopy of 63 patients with LS and 24 non-LS patients provided 40 adenomas that could be exactly matched in size and histology. In image-analysis, the CD31-positive microvessel density (2.49 {\%} vs. 2.47 {\%}, P  = 0.96), the average size of CD31-positive structures (514 μm 2 vs. 523 μm 2 , P  = 0.26) nor the amount of vascular structures per mm 2 (183 vs. 176, P  = 0.50) differed between adenomas of LS patients and non-Lynch patients. Conclusion  The outcomes of this pilot case-control study did not provide further insights into the mechanism of increased adenoma detection in LS patients using virtual chromoendoscopy techniques.",
author = "Vleugels, {Jasper L A} and {van Neerven}, {Sanne M} and {van Leerdam}, {Monique E} and Wanders, {Linda K} and {de Wit}, Meike and Beatriz Carvalho and {Delis-van Diemen}, {Pien M} and Kallenberg, {Frank G J} and Louis Vermeulen and Beli{\"e}n, {Jeroen A} and East, {James E} and Meijer, {Gerrit A} and Evelien Dekker",
year = "2019",
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language = "English",
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journal = "Endoscopy International Open",
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Vleugels, JLA, van Neerven, SM, van Leerdam, ME, Wanders, LK, de Wit, M, Carvalho, B, Delis-van Diemen, PM, Kallenberg, FGJ, Vermeulen, L, Beliën, JA, East, JE, Meijer, GA & Dekker, E 2019, 'CD31-positive microvessel density within adenomas of Lynch Syndrome patients is similar compared to adenomas of non-Lynch patients' Endoscopy International Open, vol. 7, no. 5, pp. E701-E707. https://doi.org/10.1055/a-0832-8283

CD31-positive microvessel density within adenomas of Lynch Syndrome patients is similar compared to adenomas of non-Lynch patients. / Vleugels, Jasper L A; van Neerven, Sanne M; van Leerdam, Monique E; Wanders, Linda K; de Wit, Meike; Carvalho, Beatriz; Delis-van Diemen, Pien M; Kallenberg, Frank G J; Vermeulen, Louis; Beliën, Jeroen A; East, James E; Meijer, Gerrit A; Dekker, Evelien.

In: Endoscopy International Open, Vol. 7, No. 5, 05.2019, p. E701-E707.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - CD31-positive microvessel density within adenomas of Lynch Syndrome patients is similar compared to adenomas of non-Lynch patients

AU - Vleugels, Jasper L A

AU - van Neerven, Sanne M

AU - van Leerdam, Monique E

AU - Wanders, Linda K

AU - de Wit, Meike

AU - Carvalho, Beatriz

AU - Delis-van Diemen, Pien M

AU - Kallenberg, Frank G J

AU - Vermeulen, Louis

AU - Beliën, Jeroen A

AU - East, James E

AU - Meijer, Gerrit A

AU - Dekker, Evelien

PY - 2019/5

Y1 - 2019/5

N2 - Background and study aims  Microsatellite instability accelerates colorectal cancer development in patients with Lynch syndrome (LS). Previous research showed that virtual chromoendoscopy increases detection of adenomas during colonoscopy surveillance of patients with LS. Because previous research revealed that Lynch patients have an increased vascular network in the oral mucosa, we hypothesized that increased vascularization of LS-associated adenomas is the cause of better detection with virtual chromoendoscopy. Patients and methods  In this pilot study, patients with LS having a proven germline mutation were selected from two tertiary referral hospitals and non-LS patients from an outpatient colonoscopy center. Adenomas from patients with LS were exactly matched in size and histology with adenomas from non-LS patients. Initial adenoma diagnosis was confirmed by a specialist pathologist. All adenomas were stained with CD31 and adenomatous tissue was annotated by the specialist pathologist. Image analysis of CD31-positive microvessel density was conducted using FIJI software. Results  Colonoscopy of 63 patients with LS and 24 non-LS patients provided 40 adenomas that could be exactly matched in size and histology. In image-analysis, the CD31-positive microvessel density (2.49 % vs. 2.47 %, P  = 0.96), the average size of CD31-positive structures (514 μm 2 vs. 523 μm 2 , P  = 0.26) nor the amount of vascular structures per mm 2 (183 vs. 176, P  = 0.50) differed between adenomas of LS patients and non-Lynch patients. Conclusion  The outcomes of this pilot case-control study did not provide further insights into the mechanism of increased adenoma detection in LS patients using virtual chromoendoscopy techniques.

AB - Background and study aims  Microsatellite instability accelerates colorectal cancer development in patients with Lynch syndrome (LS). Previous research showed that virtual chromoendoscopy increases detection of adenomas during colonoscopy surveillance of patients with LS. Because previous research revealed that Lynch patients have an increased vascular network in the oral mucosa, we hypothesized that increased vascularization of LS-associated adenomas is the cause of better detection with virtual chromoendoscopy. Patients and methods  In this pilot study, patients with LS having a proven germline mutation were selected from two tertiary referral hospitals and non-LS patients from an outpatient colonoscopy center. Adenomas from patients with LS were exactly matched in size and histology with adenomas from non-LS patients. Initial adenoma diagnosis was confirmed by a specialist pathologist. All adenomas were stained with CD31 and adenomatous tissue was annotated by the specialist pathologist. Image analysis of CD31-positive microvessel density was conducted using FIJI software. Results  Colonoscopy of 63 patients with LS and 24 non-LS patients provided 40 adenomas that could be exactly matched in size and histology. In image-analysis, the CD31-positive microvessel density (2.49 % vs. 2.47 %, P  = 0.96), the average size of CD31-positive structures (514 μm 2 vs. 523 μm 2 , P  = 0.26) nor the amount of vascular structures per mm 2 (183 vs. 176, P  = 0.50) differed between adenomas of LS patients and non-Lynch patients. Conclusion  The outcomes of this pilot case-control study did not provide further insights into the mechanism of increased adenoma detection in LS patients using virtual chromoendoscopy techniques.

U2 - 10.1055/a-0832-8283

DO - 10.1055/a-0832-8283

M3 - Article

VL - 7

SP - E701-E707

JO - Endoscopy International Open

JF - Endoscopy International Open

SN - 2364-3722

IS - 5

ER -