CD9 and ITGA3 are regulated during HIV-1 infection in macrophages to support viral replication

Zita Kruize, Viviana Cobos Jiménez, Fernando O. Martinez, Riccardo Di Vincenzo, Karel A. van Dort, Ad C. van Nuenen, Thijs Booiman, Neeltje A. Kootstra*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Monocytes/macrophages are important target cells for HIV-1. Here, we investigated whether HIV-1 induces changes in the macrophage gene expression profile to support viral replication. We observed that the macrophage gene expression profiles dramatically changed upon HIV-1 infection. The majority of the HIV-1 regulated genes were also differentially expressed in M2a macrophages. The biological functions associated with the HIV-1 induced gene expression profile in macrophages were mainly related to inflammatory responses. CD9 and ITGA3 were among the top genes upregulated upon HIV-1 infection. We showed that these genes support viral replication and that downregulation of these genes decreased HIV-1 replication in macrophages. Here we showed that HIV-1 infection of macrophages induces a gene expression profile that may dampen inflammatory responses. CD9 and ITGA3 were among the top genes regulated by HIV-1 and were shown to support viral production most likely at the level of viral budding and release.

Original languageEnglish
Pages (from-to)9-18
Number of pages10
Publication statusPublished - Oct 2021

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