Cerebral microdialysis of interleukin (IL)-1beta and IL-6: extraction efficiency and production in the acute phase after severe traumatic brain injury in rats

H. Folkersma, J.J.P. Breve, F.J.H. Tilders, L. Cherian, C.S. Robertson, W.P. Vandertop

Research output: Contribution to journalArticleAcademicpeer-review


BACKGROUND: As a research tool, cerebral microdialysis might be a useful technique in monitoring the release of cytokines into the extracellular fluid (ECF) following traumatic brain injury (TBI). We established extraction efficiency of Interleukin(IL)-1ss and Interleukin(IL)-6 by an in vitro microdialysis-perfusion system, followed by in vivo determination of the temporal profile of extracellular fluid cytokines after severe TBI in rats.

MATERIALS AND METHODS: In vitro experiments using a polyether sulfon (PES) microdialysis probe especially developed for recovery of macromolecules such as cytokines, were carried out to establish the extraction efficiency of IL-1ss and IL-6 from artificial cerebrospinal fluid (CSF) with defined IL-1ss and IL-6 concentrations. In vivo experiments in which rats were subjected to TBI or sham and microdialysis samples were collected from the parietal lobe for measurement of cytokines.

FINDINGS: The extraction efficiency was maximal 6.05% (range, 5.97-6.13%) at 0.5 microl/min(-1) and decreased at higher flow rates. Both cytokines were detectable in the dialysates. Highest IL-1ss levels were found within 200 min, highest IL-6 concentrations were detected at later intervals (200-400 min). No differences were found between the TBI and control groups.

CONCLUSIONS: Cerebral microdialysis allows measurement of cytokine secretion in the ECF of brain tissue in rats.

Original languageEnglish
Pages (from-to)1277-1284
JournalActa Neurochirurgica
Issue number12
Publication statusPublished - Dec 2008

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