Changes over lactation in breast milk serum proteins involved in the maturation of immune and digestive system of the infant

L. N. Zhang, M. de Waard, H. Verheijen, S. Boeren, J. A. Hageman, T. van Hooijdonk, A.J. Vervoort, J. B. van Goudoever, K. Hettinga

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

To objective of this study was to better understand the biological functions of breast milk proteins in relation to the growth and development of infants over the first six months of life. Breast milk samples from four individual women collected at seven time points in the first six months after delivery were analyzed by filter aided sample preparation and dimethyl labeling combined with liquid chromatography tandem mass spectrometry. A total of 247 and 200 milk serum proteins were identified and quantified, respectively. The milk serum proteome showed a high similarity (80% overlap) on the qualitative level between women and over lactation. The quantitative changes in milk serum proteins were mainly caused by three groups of proteins, enzymes, and transport and immunity proteins. Of these 21 significantly changed proteins, 30% were transport proteins, such as serum albumin and fatty acid binding protein, which are both involved in transporting nutrients to the infant. The decrease of the enzyme bile salt-activated lipase as well as the immunity proteins immunoglobulins and lactoferrin coincide with the gradual maturation of the digestive and immune system of infants. The human milk serum proteome didn't differ qualitatively but it did quantitatively, both between mothers and as lactation advanced. The changes of the breast milk serum proteome over lactation corresponded with the development of the digestive and immune system of infants. Biological significance: Breast milk proteins provide nutrition, but also contribute to healthy development of infants. Despite the previously reported large number of identified breast milk proteins and their changes over lactation, less is known on the changes of these proteins in individual mothers. This study is the first to determine the qualitative and quantitative changes of milk proteome over lactation between individual mothers. The results indicate that the differences in the milk proteome between individual mothers are more related to the quantitative level than qualitative level. The correlation between the changes of milk proteins and the gradual maturation of the gastrointestinal tract and immune system in infants, contributes to a better understanding of the biological functions of human milk proteins for the growth and development of infants. (C) 2016 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)40-47
Number of pages8
JournalJournal of Proteomics
Volume147
DOIs
Publication statusPublished - 2016

Cite this

Zhang, L. N. ; de Waard, M. ; Verheijen, H. ; Boeren, S. ; Hageman, J. A. ; van Hooijdonk, T. ; Vervoort, A.J. ; van Goudoever, J. B. ; Hettinga, K. / Changes over lactation in breast milk serum proteins involved in the maturation of immune and digestive system of the infant. In: Journal of Proteomics. 2016 ; Vol. 147. pp. 40-47.
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abstract = "To objective of this study was to better understand the biological functions of breast milk proteins in relation to the growth and development of infants over the first six months of life. Breast milk samples from four individual women collected at seven time points in the first six months after delivery were analyzed by filter aided sample preparation and dimethyl labeling combined with liquid chromatography tandem mass spectrometry. A total of 247 and 200 milk serum proteins were identified and quantified, respectively. The milk serum proteome showed a high similarity (80{\%} overlap) on the qualitative level between women and over lactation. The quantitative changes in milk serum proteins were mainly caused by three groups of proteins, enzymes, and transport and immunity proteins. Of these 21 significantly changed proteins, 30{\%} were transport proteins, such as serum albumin and fatty acid binding protein, which are both involved in transporting nutrients to the infant. The decrease of the enzyme bile salt-activated lipase as well as the immunity proteins immunoglobulins and lactoferrin coincide with the gradual maturation of the digestive and immune system of infants. The human milk serum proteome didn't differ qualitatively but it did quantitatively, both between mothers and as lactation advanced. The changes of the breast milk serum proteome over lactation corresponded with the development of the digestive and immune system of infants. Biological significance: Breast milk proteins provide nutrition, but also contribute to healthy development of infants. Despite the previously reported large number of identified breast milk proteins and their changes over lactation, less is known on the changes of these proteins in individual mothers. This study is the first to determine the qualitative and quantitative changes of milk proteome over lactation between individual mothers. The results indicate that the differences in the milk proteome between individual mothers are more related to the quantitative level than qualitative level. The correlation between the changes of milk proteins and the gradual maturation of the gastrointestinal tract and immune system in infants, contributes to a better understanding of the biological functions of human milk proteins for the growth and development of infants. (C) 2016 Elsevier B.V. All rights reserved.",
author = "Zhang, {L. N.} and {de Waard}, M. and H. Verheijen and S. Boeren and Hageman, {J. A.} and {van Hooijdonk}, T. and A.J. Vervoort and {van Goudoever}, {J. B.} and K. Hettinga",
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Changes over lactation in breast milk serum proteins involved in the maturation of immune and digestive system of the infant. / Zhang, L. N.; de Waard, M.; Verheijen, H.; Boeren, S.; Hageman, J. A.; van Hooijdonk, T.; Vervoort, A.J.; van Goudoever, J. B.; Hettinga, K.

In: Journal of Proteomics, Vol. 147, 2016, p. 40-47.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Changes over lactation in breast milk serum proteins involved in the maturation of immune and digestive system of the infant

AU - Zhang, L. N.

AU - de Waard, M.

AU - Verheijen, H.

AU - Boeren, S.

AU - Hageman, J. A.

AU - van Hooijdonk, T.

AU - Vervoort, A.J.

AU - van Goudoever, J. B.

AU - Hettinga, K.

N1 - ISI Document Delivery No.: DW8YX Times Cited: 2 Cited Reference Count: 47 Zhang, Lina de Waard, Marita Verheijen, Hester Boeren, Sjef Hageman, Jos A. van Hooijdonk, Toon Vervoort, Jacques van Goudoever, Johannes B. Hettinga, Kasper 2 4 6 ELSEVIER SCIENCE BV AMSTERDAM J PROTEOMICS SI

PY - 2016

Y1 - 2016

N2 - To objective of this study was to better understand the biological functions of breast milk proteins in relation to the growth and development of infants over the first six months of life. Breast milk samples from four individual women collected at seven time points in the first six months after delivery were analyzed by filter aided sample preparation and dimethyl labeling combined with liquid chromatography tandem mass spectrometry. A total of 247 and 200 milk serum proteins were identified and quantified, respectively. The milk serum proteome showed a high similarity (80% overlap) on the qualitative level between women and over lactation. The quantitative changes in milk serum proteins were mainly caused by three groups of proteins, enzymes, and transport and immunity proteins. Of these 21 significantly changed proteins, 30% were transport proteins, such as serum albumin and fatty acid binding protein, which are both involved in transporting nutrients to the infant. The decrease of the enzyme bile salt-activated lipase as well as the immunity proteins immunoglobulins and lactoferrin coincide with the gradual maturation of the digestive and immune system of infants. The human milk serum proteome didn't differ qualitatively but it did quantitatively, both between mothers and as lactation advanced. The changes of the breast milk serum proteome over lactation corresponded with the development of the digestive and immune system of infants. Biological significance: Breast milk proteins provide nutrition, but also contribute to healthy development of infants. Despite the previously reported large number of identified breast milk proteins and their changes over lactation, less is known on the changes of these proteins in individual mothers. This study is the first to determine the qualitative and quantitative changes of milk proteome over lactation between individual mothers. The results indicate that the differences in the milk proteome between individual mothers are more related to the quantitative level than qualitative level. The correlation between the changes of milk proteins and the gradual maturation of the gastrointestinal tract and immune system in infants, contributes to a better understanding of the biological functions of human milk proteins for the growth and development of infants. (C) 2016 Elsevier B.V. All rights reserved.

AB - To objective of this study was to better understand the biological functions of breast milk proteins in relation to the growth and development of infants over the first six months of life. Breast milk samples from four individual women collected at seven time points in the first six months after delivery were analyzed by filter aided sample preparation and dimethyl labeling combined with liquid chromatography tandem mass spectrometry. A total of 247 and 200 milk serum proteins were identified and quantified, respectively. The milk serum proteome showed a high similarity (80% overlap) on the qualitative level between women and over lactation. The quantitative changes in milk serum proteins were mainly caused by three groups of proteins, enzymes, and transport and immunity proteins. Of these 21 significantly changed proteins, 30% were transport proteins, such as serum albumin and fatty acid binding protein, which are both involved in transporting nutrients to the infant. The decrease of the enzyme bile salt-activated lipase as well as the immunity proteins immunoglobulins and lactoferrin coincide with the gradual maturation of the digestive and immune system of infants. The human milk serum proteome didn't differ qualitatively but it did quantitatively, both between mothers and as lactation advanced. The changes of the breast milk serum proteome over lactation corresponded with the development of the digestive and immune system of infants. Biological significance: Breast milk proteins provide nutrition, but also contribute to healthy development of infants. Despite the previously reported large number of identified breast milk proteins and their changes over lactation, less is known on the changes of these proteins in individual mothers. This study is the first to determine the qualitative and quantitative changes of milk proteome over lactation between individual mothers. The results indicate that the differences in the milk proteome between individual mothers are more related to the quantitative level than qualitative level. The correlation between the changes of milk proteins and the gradual maturation of the gastrointestinal tract and immune system in infants, contributes to a better understanding of the biological functions of human milk proteins for the growth and development of infants. (C) 2016 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.jprot.2016.02.005

DO - 10.1016/j.jprot.2016.02.005

M3 - Article

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SP - 40

EP - 47

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

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