Characterization of hematopoietic GATA transcription factor expression in mouse and human dendritic cells

Maaike R Scheenstra, Vishal Salunkhe, Iris M De Cuyper, Mark Hoogenboezem, Eveline Li, Taco W Kuijpers, Timo K van den Berg, Laura Gutiérrez

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Dendritic cells (DCs) are key initiators and regulators of the immune response. The development of the DC lineage and their subsets requires an orchestrated regulation of their transcriptional program. Gata1, a transcription factor expressed in several hematopoietic cell lineages, has been recently reported to be required for mouse DC development and function. In humans, GATA1 is involved in the lineage separation between monocyte-derived DCs and Langerhans cells (LC) and loss of GATA1 results in differentiation arrest at the monocyte stage. The hematopoietic GATA factors (i.e. Gata1, Gata2, Gata3) are known to regulate each other's expression and to function consecutively throughout lineage commitment (so-called GATA switch). In humans, mutations in GATA2 are causative of MonoMAC disease, a human immunodeficiency syndrome characterized by loss of DCs, monocytes, B and NK cells. However, additional data on the expression of hematopoietic GATA factors in the DC lineage is missing. In this study, we have characterized the expression of hematopoietic GATA factors in murine and human DCs and their expression dynamics upon TLR stimulation. We found that all hematopoietic GATA factors are expressed in DCs, but identified species-specific differences in the relative expression of each GATA factor, and how their expression fluctuates upon stimulation.

Original languageEnglish
Pages (from-to)293-303
Number of pages11
JournalBlood Cells Molecules and Diseases
Volume55
Issue number4
DOIs
Publication statusPublished - Dec 2015

Cite this

Scheenstra, M. R., Salunkhe, V., De Cuyper, I. M., Hoogenboezem, M., Li, E., Kuijpers, T. W., ... Gutiérrez, L. (2015). Characterization of hematopoietic GATA transcription factor expression in mouse and human dendritic cells. Blood Cells Molecules and Diseases, 55(4), 293-303. https://doi.org/10.1016/j.bcmd.2015.07.006