Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte cultures

Arno N. Vermeulen; Jan van Deursen; Ruud H. Brakenhoff; Ton H.W. Lensen; Thivi Ponnudurai; Joseph H.E.Th Meuwissen

doi:10.1016/0166-6851(86)90027-7

Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte cultures

Arno N. Vermeulen^*, Jan van Deursen, Ruud H. Brakenhoff, Ton H.W. Lensen, Thivi Ponnudurai, Joseph H.E.Th Meuwissen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Synchronised gametocyte cultures were used to study the biosynthesis of the sexual stage target antigens (M_r 230 000, 48 000 and 25 000) for anti gamete/zygote antibodies. These antigens were shown to be synthesized during gametocyte development from day 2-3 onwards until gametogenesis occurred. After gametogenesis a 25 kDa protein was predominantly synthesized, whereas synthesis of the other target proteins was hardly detectable. The 48, 45, and 25 kDa proteins appeared to be glycosylated, in addition the 25 kDa was also acylated in that it bound [³H]palmitic acid covalently. The iso-electric point (pI) of these proteins was assessed as being 6.0 ± 0.1 (for both 48 and 45 kDa) and 5.6 ± 0.1 (for 25 kDa).

Original language	English
Pages (from-to)	155-163
Number of pages	9
Journal	Molecular and Biochemical Parasitology
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/0166-6851(86)90027-7
Publication status	Published - Aug 1986

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title = "Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte cultures",

abstract = "Synchronised gametocyte cultures were used to study the biosynthesis of the sexual stage target antigens (Mr 230 000, 48 000 and 25 000) for anti gamete/zygote antibodies. These antigens were shown to be synthesized during gametocyte development from day 2-3 onwards until gametogenesis occurred. After gametogenesis a 25 kDa protein was predominantly synthesized, whereas synthesis of the other target proteins was hardly detectable. The 48, 45, and 25 kDa proteins appeared to be glycosylated, in addition the 25 kDa was also acylated in that it bound [3H]palmitic acid covalently. The iso-electric point (pI) of these proteins was assessed as being 6.0 ± 0.1 (for both 48 and 45 kDa) and 5.6 ± 0.1 (for 25 kDa).",

keywords = "Acylation, Gametocyte synchronization, Glycosylation, Plasmodium falciparum, Western blotting",

author = "Vermeulen, {Arno N.} and {van Deursen}, Jan and Brakenhoff, {Ruud H.} and Lensen, {Ton H.W.} and Thivi Ponnudurai and Meuwissen, {Joseph H.E.Th}",

year = "1986",

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doi = "10.1016/0166-6851(86)90027-7",

language = "English",

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journal = "Molecular and Biochemical Parasitology",

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T1 - Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte cultures

AU - Vermeulen, Arno N.

AU - van Deursen, Jan

AU - Brakenhoff, Ruud H.

AU - Lensen, Ton H.W.

AU - Ponnudurai, Thivi

AU - Meuwissen, Joseph H.E.Th

PY - 1986/8

Y1 - 1986/8

N2 - Synchronised gametocyte cultures were used to study the biosynthesis of the sexual stage target antigens (Mr 230 000, 48 000 and 25 000) for anti gamete/zygote antibodies. These antigens were shown to be synthesized during gametocyte development from day 2-3 onwards until gametogenesis occurred. After gametogenesis a 25 kDa protein was predominantly synthesized, whereas synthesis of the other target proteins was hardly detectable. The 48, 45, and 25 kDa proteins appeared to be glycosylated, in addition the 25 kDa was also acylated in that it bound [3H]palmitic acid covalently. The iso-electric point (pI) of these proteins was assessed as being 6.0 ± 0.1 (for both 48 and 45 kDa) and 5.6 ± 0.1 (for 25 kDa).

AB - Synchronised gametocyte cultures were used to study the biosynthesis of the sexual stage target antigens (Mr 230 000, 48 000 and 25 000) for anti gamete/zygote antibodies. These antigens were shown to be synthesized during gametocyte development from day 2-3 onwards until gametogenesis occurred. After gametogenesis a 25 kDa protein was predominantly synthesized, whereas synthesis of the other target proteins was hardly detectable. The 48, 45, and 25 kDa proteins appeared to be glycosylated, in addition the 25 kDa was also acylated in that it bound [3H]palmitic acid covalently. The iso-electric point (pI) of these proteins was assessed as being 6.0 ± 0.1 (for both 48 and 45 kDa) and 5.6 ± 0.1 (for 25 kDa).

KW - Acylation

KW - Gametocyte synchronization

KW - Glycosylation

KW - Plasmodium falciparum

KW - Western blotting

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SN - 0166-6851

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ER -

Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte cultures

Abstract

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