Abstract
Hippocampal pathology was shown to be extensive in multiple sclerosis (MS) and is associated with memory impairment. In this post-mortem study, we investigated hippocampal tissue from MS and Alzheimer's disease (AD) patients and compared these to non-neurological controls. By means of biochemical assessment, (immuno)histochemistry and western blot analyses, we detected substantial alterations in the cholinergic neurotransmitter system in the MS hippocampus, which were different from those in AD hippocampus. In MS hippocampus, activity and protein expression of choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme, was decreased, while the activity and protein expression of acetylcholinesterase (AChE), the acetylcholine degrading enzyme, was found to be unaltered. In contrast, in AD hippocampus both ChAT and AChE enzyme activity and protein expression was decreased. Our findings reveal an MS-specific cholinergic imbalance in the hippocampus, which may be instrumental in terms of future treatment options for memory problems in this disease.
| Original language | English |
|---|---|
| Pages (from-to) | 313-322 |
| Number of pages | 10 |
| Journal | Acta Neuropathologica |
| Volume | 122 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2011 |
Cite this
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Cholinergic imbalance in the multiple sclerosis hippocampus. / Kooi, Evert-Jan; Prins, Marloes; Bajic, Natasha; Beliën, Jeroen A M; Gerritsen, Wouter H; van Horssen, Jack; Aronica, Eleonora; van Dam, Anne-Marie; Hoozemans, Jeroen J M; Francis, Paul T; van der Valk, Paul; Geurts, Jeroen J G.
In: Acta Neuropathologica, Vol. 122, No. 3, 09.2011, p. 313-322.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Cholinergic imbalance in the multiple sclerosis hippocampus
AU - Kooi, Evert-Jan
AU - Prins, Marloes
AU - Bajic, Natasha
AU - Beliën, Jeroen A M
AU - Gerritsen, Wouter H
AU - van Horssen, Jack
AU - Aronica, Eleonora
AU - van Dam, Anne-Marie
AU - Hoozemans, Jeroen J M
AU - Francis, Paul T
AU - van der Valk, Paul
AU - Geurts, Jeroen J G
PY - 2011/9
Y1 - 2011/9
N2 - Hippocampal pathology was shown to be extensive in multiple sclerosis (MS) and is associated with memory impairment. In this post-mortem study, we investigated hippocampal tissue from MS and Alzheimer's disease (AD) patients and compared these to non-neurological controls. By means of biochemical assessment, (immuno)histochemistry and western blot analyses, we detected substantial alterations in the cholinergic neurotransmitter system in the MS hippocampus, which were different from those in AD hippocampus. In MS hippocampus, activity and protein expression of choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme, was decreased, while the activity and protein expression of acetylcholinesterase (AChE), the acetylcholine degrading enzyme, was found to be unaltered. In contrast, in AD hippocampus both ChAT and AChE enzyme activity and protein expression was decreased. Our findings reveal an MS-specific cholinergic imbalance in the hippocampus, which may be instrumental in terms of future treatment options for memory problems in this disease.
AB - Hippocampal pathology was shown to be extensive in multiple sclerosis (MS) and is associated with memory impairment. In this post-mortem study, we investigated hippocampal tissue from MS and Alzheimer's disease (AD) patients and compared these to non-neurological controls. By means of biochemical assessment, (immuno)histochemistry and western blot analyses, we detected substantial alterations in the cholinergic neurotransmitter system in the MS hippocampus, which were different from those in AD hippocampus. In MS hippocampus, activity and protein expression of choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme, was decreased, while the activity and protein expression of acetylcholinesterase (AChE), the acetylcholine degrading enzyme, was found to be unaltered. In contrast, in AD hippocampus both ChAT and AChE enzyme activity and protein expression was decreased. Our findings reveal an MS-specific cholinergic imbalance in the hippocampus, which may be instrumental in terms of future treatment options for memory problems in this disease.
KW - Acetylcholinesterase
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Alzheimer Disease
KW - Choline O-Acetyltransferase
KW - Female
KW - Hippocampus
KW - Humans
KW - Male
KW - Middle Aged
KW - Multiple Sclerosis
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1007/s00401-011-0849-4
DO - 10.1007/s00401-011-0849-4
M3 - Article
VL - 122
SP - 313
EP - 322
JO - Acta Neuropathologica
JF - Acta Neuropathologica
SN - 0001-6322
IS - 3
ER -