Claudin-1, Claudin-2 and Claudin-11 Genes Differentially Associate with Distinct Types of Anti-inflammatory Macrophages In vitro and with Parasite- and Tumour-elicited Macrophages In vivo

J. van den Bossche, D. Laoui, Y. Morias, K. Movahedi, G. Raes, P. de Baetselier, J. A. van Ginderachter*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Macrophages altered by various Th2-associated and anti-inflammatory mediators - including IL-4 and IL-13 [inducing alternatively activated macrophages (AAMs)], IL-10 and TGF-β- were generically termed M2. However, markers that discriminate between AAMs and other M2 remain scarce. We previously described E-cadherin as a marker for AAMs, permitting these macrophages to fuse upon IL-4 stimulation. To identify novel potential contributors to macrophage fusion, we assessed the effect of IL-4 on other adherens and tight junction-associated components. We observed an induction of claudin-1 (Cldn1), Cldn2 and Cldn11 genes by IL-4 in different mouse macrophage populations. Extending our findings to other stimuli revealed Cldn1 as a mainly TGF-β-induced gene and showed that Cldn11 is predominantly associated with IL-4-induced AAMs. Cldn2 is upregulated by diverse stimuli and is not associated with a specific macrophage activation state in vitro. Interestingly, different claudin genes preferentially associate with M2 from distinct diseases. While Cldn11 is predominantly expressed in AAMs from helminth-infected mice, Cldn1 is the major macrophage claudin during chronic trypanosomiasis and Cldn2 dominates in tumour-associated macrophages. Overall, we identified Cldn1, Cldn2 and Cldn11 as genes that discriminate between diverse types of M2.

Original languageEnglish
Pages (from-to)588-598
Number of pages11
JournalScandinavian Journal of Immunology
Volume75
Issue number6
DOIs
Publication statusPublished - 1 Jun 2012

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