Combination 186Re-HEDP and cisplatin supra-additive treatment effects in prostate cancer cells

Albert A. Geldof*, Laurens De Rooij, Richard T. Versteegh, Donald W.W. Newling, Gerrit J.J. Teule

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Radionuclide therapy has proven to be an efficacious palliative treatment for metastatic prostate cancer. Its potential therapeutic possibilities may be substantially increased by combining it with effective radiosensitizing drugs. Methods: This study explores the radiosensitizing properties of cisplatin when combined with 186Re-labeled hydroxyethylidene diphosphonate (HEDP) in the treatment of R3327-MATLyLu prostate cancer cells in vitro. A concomitant incubation during 4 d, combining various concentrations of cisplatin (0, 0.42, 0.83 and 1.67μmol/L) and 186Re- HEDP (0, 1.84 and 3.69 MBq/mL [0, 50 and 100 μCi/mL, respectively]) was followed by the determination of the cell numbers surviving and the replating of these cells in semisolid agar. Results: The surviving fraction of clonogenic tumor cells after combination treatment clearly showed synergism when analyzed by a panel of three different published analytical methods. In addition, analysis of variance demonstrated a significant interaction between radionuclide therapy and cisplatin-based chemotherapy (P < 0.001). Treatment with 186Re-HEDP and cisplatin by sequential incubation yielded similar, but never superior results. Conclusion: It is concluded that radionuclide therapy in combination with cisplatin is able, in principle, to improve therapeutic success rate in metastatic prostate cancer in a more than additive way.

Original languageEnglish
Pages (from-to)667-671
Number of pages5
JournalJournal of Nuclear Medicine
Volume40
Issue number4
Publication statusPublished - 1 Apr 1999

Cite this

Geldof, A. A., De Rooij, L., Versteegh, R. T., Newling, D. W. W., & Teule, G. J. J. (1999). Combination 186Re-HEDP and cisplatin supra-additive treatment effects in prostate cancer cells. Journal of Nuclear Medicine, 40(4), 667-671.