Comparative studies on the heat-induced thermotolerance of protein synthesis and cell division in synchronized mouse neuroblastoma cells

Guus Van Dongen, Louis Van De Zande, Dennis Schamhart, Roeland Van Wijk

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Mouse neuroblastoma (N2A) cells react to a heat treatment by inhibition of DNA and protein synthesis and induction of cell cycle progression delay. Mitotic delay of heat-treated G1 cells correlates with reduction of protein synthesis and is due to an extensive delay of entrance into S phase, while the G2 phase of these cells is shortened. Mitotic delay of heat-treated G2 cells is more than in G1 cells and no correlation with protein synthesis reduction is found. In heat-treated G1 phase cells, both protein synthesis and cell cycle progression become thermotolerant to a second incubation at increased temperature. Moreover, the process of DNA synthesis becomes thermotolerant. In contrast, when heat-treated G1 phase cells have progressed into G2 phase and are then incubated at increased temperature, this G2 phase delay is not diminished. Apparently, additional targets for hyperthermia are present in late S and G2 phase cells.

Original languageEnglish
Pages (from-to)759-769
Number of pages11
JournalInternational Journal of Radiation Biology
Volume46
Issue number6
DOIs
Publication statusPublished - 1 Jan 1984

Cite this

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title = "Comparative studies on the heat-induced thermotolerance of protein synthesis and cell division in synchronized mouse neuroblastoma cells",
abstract = "Mouse neuroblastoma (N2A) cells react to a heat treatment by inhibition of DNA and protein synthesis and induction of cell cycle progression delay. Mitotic delay of heat-treated G1 cells correlates with reduction of protein synthesis and is due to an extensive delay of entrance into S phase, while the G2 phase of these cells is shortened. Mitotic delay of heat-treated G2 cells is more than in G1 cells and no correlation with protein synthesis reduction is found. In heat-treated G1 phase cells, both protein synthesis and cell cycle progression become thermotolerant to a second incubation at increased temperature. Moreover, the process of DNA synthesis becomes thermotolerant. In contrast, when heat-treated G1 phase cells have progressed into G2 phase and are then incubated at increased temperature, this G2 phase delay is not diminished. Apparently, additional targets for hyperthermia are present in late S and G2 phase cells.",
keywords = "Hyperthermia, Mitotic delay, Protein synthesis, Thermo-tolerance",
author = "{Van Dongen}, Guus and {Van De Zande}, Louis and Dennis Schamhart and {Van Wijk}, Roeland",
year = "1984",
month = "1",
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volume = "46",
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}

Comparative studies on the heat-induced thermotolerance of protein synthesis and cell division in synchronized mouse neuroblastoma cells. / Van Dongen, Guus; Van De Zande, Louis; Schamhart, Dennis; Van Wijk, Roeland.

In: International Journal of Radiation Biology, Vol. 46, No. 6, 01.01.1984, p. 759-769.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Comparative studies on the heat-induced thermotolerance of protein synthesis and cell division in synchronized mouse neuroblastoma cells

AU - Van Dongen, Guus

AU - Van De Zande, Louis

AU - Schamhart, Dennis

AU - Van Wijk, Roeland

PY - 1984/1/1

Y1 - 1984/1/1

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AB - Mouse neuroblastoma (N2A) cells react to a heat treatment by inhibition of DNA and protein synthesis and induction of cell cycle progression delay. Mitotic delay of heat-treated G1 cells correlates with reduction of protein synthesis and is due to an extensive delay of entrance into S phase, while the G2 phase of these cells is shortened. Mitotic delay of heat-treated G2 cells is more than in G1 cells and no correlation with protein synthesis reduction is found. In heat-treated G1 phase cells, both protein synthesis and cell cycle progression become thermotolerant to a second incubation at increased temperature. Moreover, the process of DNA synthesis becomes thermotolerant. In contrast, when heat-treated G1 phase cells have progressed into G2 phase and are then incubated at increased temperature, this G2 phase delay is not diminished. Apparently, additional targets for hyperthermia are present in late S and G2 phase cells.

KW - Hyperthermia

KW - Mitotic delay

KW - Protein synthesis

KW - Thermo-tolerance

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