Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

A. T. Hesselink, A. J.C. Van Den Brule, Z. M.A. Groothuismink, M. Molano, J. Berkhof, C. J.L.M. Meijer, P. J.F. Snijders*

*Corresponding author for this work

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Abstract

We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

Original languageEnglish
Pages (from-to)4868-4871
Number of pages4
JournalJournal of Clinical Microbiology
Volume43
Issue number9
DOIs
Publication statusPublished - 1 Sep 2005

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