TY - JOUR
T1 - Compromising human skin in vivo and ex vivo to study skin barrier repair
AU - Berkers, T.
AU - Boiten, W. A.
AU - Absalah, S.
AU - van Smeden, J.
AU - Lavrijsen, A. P.M.
AU - Bouwstra, J. A.
N1 - Funding Information:
The authors would like acknowledge Gert Gooris for his help with programming the FTIR method. The authors express their gratitude to Evonik for supplying the synthetic CERs. This research was financially supported by Dutch Technology Foundation TTW (grant no. 12400 ).
Publisher Copyright:
© 2019 The Authors
PY - 2019/8
Y1 - 2019/8
N2 - Ex vivo regenerated stratum corneum (SC)after tape-stripping can be used as a model to study the barrier function of compromised skin. Yet, details about how close the regenerated SC model mimics the lipid properties (e.g. lipid composition and lipid ordering)of the in vivo situation are not known. Here, we examined using a comprehensive ceramide analysis whether human ex vivo regenerated SC showed similar lipid properties as human in vivo regenerated SC. Both in vivo and ex vivo regenerated SC had an altered ceramide subclass composition, with increased percentages of sphingosine-based subclass and decreased percentages of phytosphingosine-based subclass ceramides, a reduced mean ceramide chain length, and a higher percentage of unsaturated ceramides. Overall, regenerated SC ex vivo showed more pronounced but similar changes compared to the in vivo response. One of the purposes of these models is to use them to mimic compromised skin of inflammatory skin diseases. The altered lipid properties in regenerated SC were comparable to those observed in several inflammatory skin diseases, which makes them a valuable model for the barrier properties in inflammatory skin diseases.
AB - Ex vivo regenerated stratum corneum (SC)after tape-stripping can be used as a model to study the barrier function of compromised skin. Yet, details about how close the regenerated SC model mimics the lipid properties (e.g. lipid composition and lipid ordering)of the in vivo situation are not known. Here, we examined using a comprehensive ceramide analysis whether human ex vivo regenerated SC showed similar lipid properties as human in vivo regenerated SC. Both in vivo and ex vivo regenerated SC had an altered ceramide subclass composition, with increased percentages of sphingosine-based subclass and decreased percentages of phytosphingosine-based subclass ceramides, a reduced mean ceramide chain length, and a higher percentage of unsaturated ceramides. Overall, regenerated SC ex vivo showed more pronounced but similar changes compared to the in vivo response. One of the purposes of these models is to use them to mimic compromised skin of inflammatory skin diseases. The altered lipid properties in regenerated SC were comparable to those observed in several inflammatory skin diseases, which makes them a valuable model for the barrier properties in inflammatory skin diseases.
UR - http://www.scopus.com/inward/record.url?scp=85064767793&partnerID=8YFLogxK
U2 - 10.1016/j.bbalip.2019.04.005
DO - 10.1016/j.bbalip.2019.04.005
M3 - Article
C2 - 31002944
AN - SCOPUS:85064767793
SN - 1388-1981
VL - 1864
SP - 1103
EP - 1108
JO - Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
IS - 8
ER -