Core alpha1-->3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep

I van Die, V Gomord, F N Kooyman, T K van den Berg, R D Cummings, L Vervelde

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Synthesis of parasite specific IgE plays a critical role in the defence against helminth infections. We report here that IgE from serum from Schistosoma mansoni infected mice and Haemonchus contortus infected sheep recognizes complex-type N-glycans from Arabidopsis thaliana, which contain R-GlcNAcbeta1-->4(Fucalpha1-->3)GlcNAcbeta1-Asn (core alpha1-->3-Fuc) and Xylbeta1-->2Manbeta1-->4GlcNAcbeta1-R (core beta1-->2-Xyl) modifications, and honeybee phospholipase A2, which carries N-glycans that contain the core alpha1-->3-Fuc epitope. Evidence is presented that core alpha1-->3-fucosylated N-glycans bind a substantial part of the parasite specific IgE in serum of H. contortus infected sheep. These results suggest that the core alpha1-->3-Fuc antigen may contribute to induction of a Th2 response leading to the production of IgE. In addition we show here that N-glycans carrying core alpha1-->3-Fuc and beta1-->2-Xyl antigens are synthesized by many parasitic helminths and also by the free living nematode Caenorhabditis elegans. Since N-glycans containing the core alpha1-->3-Fuc have also been implicated in honeybee and plant induced allergies, this conserved glycan might represent an important common IgE epitope.

Original languageEnglish
Pages (from-to)189-93
Number of pages5
JournalFEBS Letters
Volume463
Issue number1-2
Publication statusPublished - 10 Dec 1999

Cite this

@article{146072a7ca3f47a0a7127734c9f8dd83,
title = "Core alpha1-->3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep",
abstract = "Synthesis of parasite specific IgE plays a critical role in the defence against helminth infections. We report here that IgE from serum from Schistosoma mansoni infected mice and Haemonchus contortus infected sheep recognizes complex-type N-glycans from Arabidopsis thaliana, which contain R-GlcNAcbeta1-->4(Fucalpha1-->3)GlcNAcbeta1-Asn (core alpha1-->3-Fuc) and Xylbeta1-->2Manbeta1-->4GlcNAcbeta1-R (core beta1-->2-Xyl) modifications, and honeybee phospholipase A2, which carries N-glycans that contain the core alpha1-->3-Fuc epitope. Evidence is presented that core alpha1-->3-fucosylated N-glycans bind a substantial part of the parasite specific IgE in serum of H. contortus infected sheep. These results suggest that the core alpha1-->3-Fuc antigen may contribute to induction of a Th2 response leading to the production of IgE. In addition we show here that N-glycans carrying core alpha1-->3-Fuc and beta1-->2-Xyl antigens are synthesized by many parasitic helminths and also by the free living nematode Caenorhabditis elegans. Since N-glycans containing the core alpha1-->3-Fuc have also been implicated in honeybee and plant induced allergies, this conserved glycan might represent an important common IgE epitope.",
keywords = "Animals, Arabidopsis/chemistry, Binding, Competitive, Blotting, Western, Caenorhabditis elegans/chemistry, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Epitopes/immunology, Fucose/chemistry, Haemonchiasis/immunology, Haemonchus/chemistry, Immunoglobulin E/blood, Immunoglobulin G/blood, Mice, Polysaccharides/chemistry, Schistosoma mansoni/chemistry, Sheep/immunology, Substrate Specificity",
author = "{van Die}, I and V Gomord and Kooyman, {F N} and {van den Berg}, {T K} and Cummings, {R D} and L Vervelde",
year = "1999",
month = "12",
day = "10",
language = "English",
volume = "463",
pages = "189--93",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "1-2",

}

Core alpha1-->3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep. / van Die, I; Gomord, V; Kooyman, F N; van den Berg, T K; Cummings, R D; Vervelde, L.

In: FEBS Letters, Vol. 463, No. 1-2, 10.12.1999, p. 189-93.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Core alpha1-->3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep

AU - van Die, I

AU - Gomord, V

AU - Kooyman, F N

AU - van den Berg, T K

AU - Cummings, R D

AU - Vervelde, L

PY - 1999/12/10

Y1 - 1999/12/10

N2 - Synthesis of parasite specific IgE plays a critical role in the defence against helminth infections. We report here that IgE from serum from Schistosoma mansoni infected mice and Haemonchus contortus infected sheep recognizes complex-type N-glycans from Arabidopsis thaliana, which contain R-GlcNAcbeta1-->4(Fucalpha1-->3)GlcNAcbeta1-Asn (core alpha1-->3-Fuc) and Xylbeta1-->2Manbeta1-->4GlcNAcbeta1-R (core beta1-->2-Xyl) modifications, and honeybee phospholipase A2, which carries N-glycans that contain the core alpha1-->3-Fuc epitope. Evidence is presented that core alpha1-->3-fucosylated N-glycans bind a substantial part of the parasite specific IgE in serum of H. contortus infected sheep. These results suggest that the core alpha1-->3-Fuc antigen may contribute to induction of a Th2 response leading to the production of IgE. In addition we show here that N-glycans carrying core alpha1-->3-Fuc and beta1-->2-Xyl antigens are synthesized by many parasitic helminths and also by the free living nematode Caenorhabditis elegans. Since N-glycans containing the core alpha1-->3-Fuc have also been implicated in honeybee and plant induced allergies, this conserved glycan might represent an important common IgE epitope.

AB - Synthesis of parasite specific IgE plays a critical role in the defence against helminth infections. We report here that IgE from serum from Schistosoma mansoni infected mice and Haemonchus contortus infected sheep recognizes complex-type N-glycans from Arabidopsis thaliana, which contain R-GlcNAcbeta1-->4(Fucalpha1-->3)GlcNAcbeta1-Asn (core alpha1-->3-Fuc) and Xylbeta1-->2Manbeta1-->4GlcNAcbeta1-R (core beta1-->2-Xyl) modifications, and honeybee phospholipase A2, which carries N-glycans that contain the core alpha1-->3-Fuc epitope. Evidence is presented that core alpha1-->3-fucosylated N-glycans bind a substantial part of the parasite specific IgE in serum of H. contortus infected sheep. These results suggest that the core alpha1-->3-Fuc antigen may contribute to induction of a Th2 response leading to the production of IgE. In addition we show here that N-glycans carrying core alpha1-->3-Fuc and beta1-->2-Xyl antigens are synthesized by many parasitic helminths and also by the free living nematode Caenorhabditis elegans. Since N-glycans containing the core alpha1-->3-Fuc have also been implicated in honeybee and plant induced allergies, this conserved glycan might represent an important common IgE epitope.

KW - Animals

KW - Arabidopsis/chemistry

KW - Binding, Competitive

KW - Blotting, Western

KW - Caenorhabditis elegans/chemistry

KW - Dose-Response Relationship, Drug

KW - Enzyme-Linked Immunosorbent Assay

KW - Epitopes/immunology

KW - Fucose/chemistry

KW - Haemonchiasis/immunology

KW - Haemonchus/chemistry

KW - Immunoglobulin E/blood

KW - Immunoglobulin G/blood

KW - Mice

KW - Polysaccharides/chemistry

KW - Schistosoma mansoni/chemistry

KW - Sheep/immunology

KW - Substrate Specificity

M3 - Article

VL - 463

SP - 189

EP - 193

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -