Cutaneous human papillomavirus genotypes in different kinds of skin lesions in Argentina

Rita Mariel Correa, Sara Vladimirsky, Daniëlle A M Heideman, Mauro Coringrato, Alejandra Abeldaño, Liliana Olivares, Roxana Del Aguila, Lidia Virginia Alonio, Peter J F Snijders, María Alejandra Picconi

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Cutaneous human papillomaviruses (HPVs) comprise a large and highly heterogeneous virus group. Some of the cutaneous HPVs of the genus Beta have been suggested as a co-factor in the development of non-melanoma skin cancer (NMSC). The aim of this study was to determine cutaneous HPV prevalence and type-specific distribution in different kinds of skin lesions from Argentine patients visiting Dermatology Departments of three hospitals from Buenos Aires. A cross-sectional analysis was performed. HPV DNA was analyzed in (i) 3 patients with Epidermodysplasia verruciformis (EV) harboring benign lesions (BL) (n = 1) and squamous cell carcinoma (SCC) (n = 4); (ii) 240 non-EV patients harboring: (a) BL (n = 38), (b) Actinic Keratosis (AK) (n = 83), (c) SCC (n = 74), and (d) basal cell carcinoma (BCC) (n = 96). Detection and genotyping of 35 cutaneous HPV DNA was carried out by BGC-PCR and GP5+/6 + PCR followed by reverse line blot assay. In EV patients, Beta types were found in all lesions (5/5), including the potentially high-risk HPV types 5 and 8, mostly in multiple infections. In non-EV patients, cutaneous types were found in 50.0% of BL, 43.4% of AK, 31.1% of SCC, and 16.7% of BCC. Beta HPVs were the most frequently found in all lesions, being present in all AK and SCC cases that were positive for HPV. No type-specific correlation with lesion severity was found. In our series, a wide spectrum of cutaneous HPV types was detected in different skin lesions. A possible role for these HPVs in skin carcinogenesis deserves further study. J. Med. Virol. 89:352-357, 2017. © 2016 Wiley Periodicals, Inc.

Original languageEnglish
Pages (from-to)352-357
Number of pages6
JournalJournal of Medical Virology
Volume89
Issue number2
DOIs
Publication statusPublished - Feb 2017

Cite this

Correa, R. M., Vladimirsky, S., Heideman, D. A. M., Coringrato, M., Abeldaño, A., Olivares, L., ... Picconi, M. A. (2017). Cutaneous human papillomavirus genotypes in different kinds of skin lesions in Argentina. Journal of Medical Virology, 89(2), 352-357. https://doi.org/10.1002/jmv.24631
Correa, Rita Mariel ; Vladimirsky, Sara ; Heideman, Daniëlle A M ; Coringrato, Mauro ; Abeldaño, Alejandra ; Olivares, Liliana ; Del Aguila, Roxana ; Alonio, Lidia Virginia ; Snijders, Peter J F ; Picconi, María Alejandra. / Cutaneous human papillomavirus genotypes in different kinds of skin lesions in Argentina. In: Journal of Medical Virology. 2017 ; Vol. 89, No. 2. pp. 352-357.
@article{dc1f18b7c2af469fadb6312384bc3eb3,
title = "Cutaneous human papillomavirus genotypes in different kinds of skin lesions in Argentina",
abstract = "Cutaneous human papillomaviruses (HPVs) comprise a large and highly heterogeneous virus group. Some of the cutaneous HPVs of the genus Beta have been suggested as a co-factor in the development of non-melanoma skin cancer (NMSC). The aim of this study was to determine cutaneous HPV prevalence and type-specific distribution in different kinds of skin lesions from Argentine patients visiting Dermatology Departments of three hospitals from Buenos Aires. A cross-sectional analysis was performed. HPV DNA was analyzed in (i) 3 patients with Epidermodysplasia verruciformis (EV) harboring benign lesions (BL) (n = 1) and squamous cell carcinoma (SCC) (n = 4); (ii) 240 non-EV patients harboring: (a) BL (n = 38), (b) Actinic Keratosis (AK) (n = 83), (c) SCC (n = 74), and (d) basal cell carcinoma (BCC) (n = 96). Detection and genotyping of 35 cutaneous HPV DNA was carried out by BGC-PCR and GP5+/6 + PCR followed by reverse line blot assay. In EV patients, Beta types were found in all lesions (5/5), including the potentially high-risk HPV types 5 and 8, mostly in multiple infections. In non-EV patients, cutaneous types were found in 50.0{\%} of BL, 43.4{\%} of AK, 31.1{\%} of SCC, and 16.7{\%} of BCC. Beta HPVs were the most frequently found in all lesions, being present in all AK and SCC cases that were positive for HPV. No type-specific correlation with lesion severity was found. In our series, a wide spectrum of cutaneous HPV types was detected in different skin lesions. A possible role for these HPVs in skin carcinogenesis deserves further study. J. Med. Virol. 89:352-357, 2017. {\circledC} 2016 Wiley Periodicals, Inc.",
keywords = "Aged, Argentina, Cross-Sectional Studies, Female, Genotype, Genotyping Techniques, Humans, Male, Middle Aged, Molecular Epidemiology, Nucleic Acid Hybridization, Papillomaviridae, Papillomavirus Infections, Polymerase Chain Reaction, Prevalence, Skin Diseases, Viral, Journal Article",
author = "Correa, {Rita Mariel} and Sara Vladimirsky and Heideman, {Dani{\"e}lle A M} and Mauro Coringrato and Alejandra Abelda{\~n}o and Liliana Olivares and {Del Aguila}, Roxana and Alonio, {Lidia Virginia} and Snijders, {Peter J F} and Picconi, {Mar{\'i}a Alejandra}",
note = "{\circledC} 2016 Wiley Periodicals, Inc.",
year = "2017",
month = "2",
doi = "10.1002/jmv.24631",
language = "English",
volume = "89",
pages = "352--357",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "2",

}

Correa, RM, Vladimirsky, S, Heideman, DAM, Coringrato, M, Abeldaño, A, Olivares, L, Del Aguila, R, Alonio, LV, Snijders, PJF & Picconi, MA 2017, 'Cutaneous human papillomavirus genotypes in different kinds of skin lesions in Argentina' Journal of Medical Virology, vol. 89, no. 2, pp. 352-357. https://doi.org/10.1002/jmv.24631

Cutaneous human papillomavirus genotypes in different kinds of skin lesions in Argentina. / Correa, Rita Mariel; Vladimirsky, Sara; Heideman, Daniëlle A M; Coringrato, Mauro; Abeldaño, Alejandra; Olivares, Liliana; Del Aguila, Roxana; Alonio, Lidia Virginia; Snijders, Peter J F; Picconi, María Alejandra.

In: Journal of Medical Virology, Vol. 89, No. 2, 02.2017, p. 352-357.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cutaneous human papillomavirus genotypes in different kinds of skin lesions in Argentina

AU - Correa, Rita Mariel

AU - Vladimirsky, Sara

AU - Heideman, Daniëlle A M

AU - Coringrato, Mauro

AU - Abeldaño, Alejandra

AU - Olivares, Liliana

AU - Del Aguila, Roxana

AU - Alonio, Lidia Virginia

AU - Snijders, Peter J F

AU - Picconi, María Alejandra

N1 - © 2016 Wiley Periodicals, Inc.

PY - 2017/2

Y1 - 2017/2

N2 - Cutaneous human papillomaviruses (HPVs) comprise a large and highly heterogeneous virus group. Some of the cutaneous HPVs of the genus Beta have been suggested as a co-factor in the development of non-melanoma skin cancer (NMSC). The aim of this study was to determine cutaneous HPV prevalence and type-specific distribution in different kinds of skin lesions from Argentine patients visiting Dermatology Departments of three hospitals from Buenos Aires. A cross-sectional analysis was performed. HPV DNA was analyzed in (i) 3 patients with Epidermodysplasia verruciformis (EV) harboring benign lesions (BL) (n = 1) and squamous cell carcinoma (SCC) (n = 4); (ii) 240 non-EV patients harboring: (a) BL (n = 38), (b) Actinic Keratosis (AK) (n = 83), (c) SCC (n = 74), and (d) basal cell carcinoma (BCC) (n = 96). Detection and genotyping of 35 cutaneous HPV DNA was carried out by BGC-PCR and GP5+/6 + PCR followed by reverse line blot assay. In EV patients, Beta types were found in all lesions (5/5), including the potentially high-risk HPV types 5 and 8, mostly in multiple infections. In non-EV patients, cutaneous types were found in 50.0% of BL, 43.4% of AK, 31.1% of SCC, and 16.7% of BCC. Beta HPVs were the most frequently found in all lesions, being present in all AK and SCC cases that were positive for HPV. No type-specific correlation with lesion severity was found. In our series, a wide spectrum of cutaneous HPV types was detected in different skin lesions. A possible role for these HPVs in skin carcinogenesis deserves further study. J. Med. Virol. 89:352-357, 2017. © 2016 Wiley Periodicals, Inc.

AB - Cutaneous human papillomaviruses (HPVs) comprise a large and highly heterogeneous virus group. Some of the cutaneous HPVs of the genus Beta have been suggested as a co-factor in the development of non-melanoma skin cancer (NMSC). The aim of this study was to determine cutaneous HPV prevalence and type-specific distribution in different kinds of skin lesions from Argentine patients visiting Dermatology Departments of three hospitals from Buenos Aires. A cross-sectional analysis was performed. HPV DNA was analyzed in (i) 3 patients with Epidermodysplasia verruciformis (EV) harboring benign lesions (BL) (n = 1) and squamous cell carcinoma (SCC) (n = 4); (ii) 240 non-EV patients harboring: (a) BL (n = 38), (b) Actinic Keratosis (AK) (n = 83), (c) SCC (n = 74), and (d) basal cell carcinoma (BCC) (n = 96). Detection and genotyping of 35 cutaneous HPV DNA was carried out by BGC-PCR and GP5+/6 + PCR followed by reverse line blot assay. In EV patients, Beta types were found in all lesions (5/5), including the potentially high-risk HPV types 5 and 8, mostly in multiple infections. In non-EV patients, cutaneous types were found in 50.0% of BL, 43.4% of AK, 31.1% of SCC, and 16.7% of BCC. Beta HPVs were the most frequently found in all lesions, being present in all AK and SCC cases that were positive for HPV. No type-specific correlation with lesion severity was found. In our series, a wide spectrum of cutaneous HPV types was detected in different skin lesions. A possible role for these HPVs in skin carcinogenesis deserves further study. J. Med. Virol. 89:352-357, 2017. © 2016 Wiley Periodicals, Inc.

KW - Aged

KW - Argentina

KW - Cross-Sectional Studies

KW - Female

KW - Genotype

KW - Genotyping Techniques

KW - Humans

KW - Male

KW - Middle Aged

KW - Molecular Epidemiology

KW - Nucleic Acid Hybridization

KW - Papillomaviridae

KW - Papillomavirus Infections

KW - Polymerase Chain Reaction

KW - Prevalence

KW - Skin Diseases, Viral

KW - Journal Article

U2 - 10.1002/jmv.24631

DO - 10.1002/jmv.24631

M3 - Article

VL - 89

SP - 352

EP - 357

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 2

ER -