Cutting edge: the HLA-A*0101-restricted HY minor histocompatibility antigen originates from DFFRY and contains a cysteinylated cysteine residue as identified by a novel mass spectrometric technique

R A Pierce, E D Field, J M den Haan, J A Caldwell, F M White, J A Marto, W Wang, L M Frost, E Blokland, C Reinhardus, J Shabanowitz, D F Hunt, E Goulmy, V H Engelhard

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In this report, we describe the use of novel mass spectrometry instrumentation to identify a male-specific minor histocompatibility Ag restricted by HLA-A*0101 (A1-HY). This Ag has the sequence IVDC*LTEMY, where C* represents a cysteine disulfide bonded to a second cysteine residue. The core peptide sequence is found in the protein product of DFFRY, a Y chromosome gene not previously identified as the source of an HY Ag. The male-specific form of the peptide differs from its X chromosomal counterpart by the substitution of serine for the C* residue. Both peptides are expressed on the cell surface at 30 or fewer copies per cell. However, A1-HY-specific CTL recognize the DFFRY-derived peptide at a 1500-fold lower dose than the female homologue. Thus, these studies have identified a new source of HY epitopes and provide additional information about the influence of posttranslational modifications of class I-associated peptides on T cell recognition.

Original languageEnglish
Pages (from-to)6360-4
Number of pages5
JournalJournal of Immunology
Volume163
Issue number12
Publication statusPublished - 15 Dec 1999

Cite this

Pierce, R A ; Field, E D ; den Haan, J M ; Caldwell, J A ; White, F M ; Marto, J A ; Wang, W ; Frost, L M ; Blokland, E ; Reinhardus, C ; Shabanowitz, J ; Hunt, D F ; Goulmy, E ; Engelhard, V H. / Cutting edge : the HLA-A*0101-restricted HY minor histocompatibility antigen originates from DFFRY and contains a cysteinylated cysteine residue as identified by a novel mass spectrometric technique. In: Journal of Immunology. 1999 ; Vol. 163, No. 12. pp. 6360-4.
@article{929db7edf2ba413a8e135c137256a467,
title = "Cutting edge: the HLA-A*0101-restricted HY minor histocompatibility antigen originates from DFFRY and contains a cysteinylated cysteine residue as identified by a novel mass spectrometric technique",
abstract = "In this report, we describe the use of novel mass spectrometry instrumentation to identify a male-specific minor histocompatibility Ag restricted by HLA-A*0101 (A1-HY). This Ag has the sequence IVDC*LTEMY, where C* represents a cysteine disulfide bonded to a second cysteine residue. The core peptide sequence is found in the protein product of DFFRY, a Y chromosome gene not previously identified as the source of an HY Ag. The male-specific form of the peptide differs from its X chromosomal counterpart by the substitution of serine for the C* residue. Both peptides are expressed on the cell surface at 30 or fewer copies per cell. However, A1-HY-specific CTL recognize the DFFRY-derived peptide at a 1500-fold lower dose than the female homologue. Thus, these studies have identified a new source of HY epitopes and provide additional information about the influence of posttranslational modifications of class I-associated peptides on T cell recognition.",
keywords = "Antigens, Surface/isolation & purification, Cells, Cultured, Cysteine/metabolism, Disulfides/metabolism, Epitopes/isolation & purification, Female, H-Y Antigen/isolation & purification, HLA-A Antigens/immunology, Humans, Male, Mass Spectrometry/methods, Methionine/metabolism, X Chromosome/genetics, Y Chromosome/genetics",
author = "Pierce, {R A} and Field, {E D} and {den Haan}, {J M} and Caldwell, {J A} and White, {F M} and Marto, {J A} and W Wang and Frost, {L M} and E Blokland and C Reinhardus and J Shabanowitz and Hunt, {D F} and E Goulmy and Engelhard, {V H}",
year = "1999",
month = "12",
day = "15",
language = "English",
volume = "163",
pages = "6360--4",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

Pierce, RA, Field, ED, den Haan, JM, Caldwell, JA, White, FM, Marto, JA, Wang, W, Frost, LM, Blokland, E, Reinhardus, C, Shabanowitz, J, Hunt, DF, Goulmy, E & Engelhard, VH 1999, 'Cutting edge: the HLA-A*0101-restricted HY minor histocompatibility antigen originates from DFFRY and contains a cysteinylated cysteine residue as identified by a novel mass spectrometric technique' Journal of Immunology, vol. 163, no. 12, pp. 6360-4.

Cutting edge : the HLA-A*0101-restricted HY minor histocompatibility antigen originates from DFFRY and contains a cysteinylated cysteine residue as identified by a novel mass spectrometric technique. / Pierce, R A; Field, E D; den Haan, J M; Caldwell, J A; White, F M; Marto, J A; Wang, W; Frost, L M; Blokland, E; Reinhardus, C; Shabanowitz, J; Hunt, D F; Goulmy, E; Engelhard, V H.

In: Journal of Immunology, Vol. 163, No. 12, 15.12.1999, p. 6360-4.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cutting edge

T2 - the HLA-A*0101-restricted HY minor histocompatibility antigen originates from DFFRY and contains a cysteinylated cysteine residue as identified by a novel mass spectrometric technique

AU - Pierce, R A

AU - Field, E D

AU - den Haan, J M

AU - Caldwell, J A

AU - White, F M

AU - Marto, J A

AU - Wang, W

AU - Frost, L M

AU - Blokland, E

AU - Reinhardus, C

AU - Shabanowitz, J

AU - Hunt, D F

AU - Goulmy, E

AU - Engelhard, V H

PY - 1999/12/15

Y1 - 1999/12/15

N2 - In this report, we describe the use of novel mass spectrometry instrumentation to identify a male-specific minor histocompatibility Ag restricted by HLA-A*0101 (A1-HY). This Ag has the sequence IVDC*LTEMY, where C* represents a cysteine disulfide bonded to a second cysteine residue. The core peptide sequence is found in the protein product of DFFRY, a Y chromosome gene not previously identified as the source of an HY Ag. The male-specific form of the peptide differs from its X chromosomal counterpart by the substitution of serine for the C* residue. Both peptides are expressed on the cell surface at 30 or fewer copies per cell. However, A1-HY-specific CTL recognize the DFFRY-derived peptide at a 1500-fold lower dose than the female homologue. Thus, these studies have identified a new source of HY epitopes and provide additional information about the influence of posttranslational modifications of class I-associated peptides on T cell recognition.

AB - In this report, we describe the use of novel mass spectrometry instrumentation to identify a male-specific minor histocompatibility Ag restricted by HLA-A*0101 (A1-HY). This Ag has the sequence IVDC*LTEMY, where C* represents a cysteine disulfide bonded to a second cysteine residue. The core peptide sequence is found in the protein product of DFFRY, a Y chromosome gene not previously identified as the source of an HY Ag. The male-specific form of the peptide differs from its X chromosomal counterpart by the substitution of serine for the C* residue. Both peptides are expressed on the cell surface at 30 or fewer copies per cell. However, A1-HY-specific CTL recognize the DFFRY-derived peptide at a 1500-fold lower dose than the female homologue. Thus, these studies have identified a new source of HY epitopes and provide additional information about the influence of posttranslational modifications of class I-associated peptides on T cell recognition.

KW - Antigens, Surface/isolation & purification

KW - Cells, Cultured

KW - Cysteine/metabolism

KW - Disulfides/metabolism

KW - Epitopes/isolation & purification

KW - Female

KW - H-Y Antigen/isolation & purification

KW - HLA-A Antigens/immunology

KW - Humans

KW - Male

KW - Mass Spectrometry/methods

KW - Methionine/metabolism

KW - X Chromosome/genetics

KW - Y Chromosome/genetics

M3 - Article

VL - 163

SP - 6360

EP - 6364

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 12

ER -