Abstract
Using indirect immunofluorescence and a panel of human convalescent-phase sera, we identified cytomegalovirus (CMV) early and late membrane antigens (CMV-EMA and CMV-LMA, respectively) as separate entities on the surfaces of viable CMV-infected fibroblasts starting at 6 to 12 and 36 to 48 h postinoculation, respectively. For expression of CMV-EMA and CMV-LMA, infectious virus and active protein synthesis were required, whereas the expression of CMV-LMA, in addition, required viral DNA synthesis. Our data suggest that CMV-EMA and CMV-LMA form an individual set of CMV antigens that are different from intracellular CMV antigens and possibly (partly) different from the viral envelope.
| Original language | English |
|---|---|
| Pages (from-to) | 240-4 |
| Number of pages | 5 |
| Journal | Journal of Virology |
| Volume | 54 |
| Issue number | 1 |
| Publication status | Published - Apr 1985 |
Cite this
Middeldorp, J. M., Jongsma, J., & The, T. H. (1985). Cytomegalovirus early and late membrane antigens detected by antibodies in human convalescent sera. Journal of Virology, 54(1), 240-4.