DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells

T B Geijtenbeek, D S Kwon, R Torensma, S J van Vliet, G C van Duijnhoven, J Middel, I L Cornelissen, H S Nottet, V N KewalRamani, D R Littman, C G Figdor, Y van Kooyk

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of a DC-specific C-type lectin, DC-SIGN, that is highly expressed on DC present in mucosal tissues and binds to the HIV-1 envelope glycoprotein gp120. DC-SIGN does not function as a receptor for viral entry into DC but instead promotes efficient infection in trans of cells that express CD4 and chemokine receptors. We propose that DC-SIGN efficiently captures HIV-1 in the periphery and facilitates its transport to secondary lymphoid organs rich in T cells, to enhance infection in trans of these target cells.

Original languageEnglish
Pages (from-to)587-97
Number of pages11
JournalCell
Volume100
Issue number5
Publication statusPublished - 3 Mar 2000

Cite this

Geijtenbeek, T. B., Kwon, D. S., Torensma, R., van Vliet, S. J., van Duijnhoven, G. C., Middel, J., ... van Kooyk, Y. (2000). DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell, 100(5), 587-97.
Geijtenbeek, T B ; Kwon, D S ; Torensma, R ; van Vliet, S J ; van Duijnhoven, G C ; Middel, J ; Cornelissen, I L ; Nottet, H S ; KewalRamani, V N ; Littman, D R ; Figdor, C G ; van Kooyk, Y. / DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. In: Cell. 2000 ; Vol. 100, No. 5. pp. 587-97.
@article{e0c81d0409864c0f821f1c71625ac6c8,
title = "DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells",
abstract = "Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of a DC-specific C-type lectin, DC-SIGN, that is highly expressed on DC present in mucosal tissues and binds to the HIV-1 envelope glycoprotein gp120. DC-SIGN does not function as a receptor for viral entry into DC but instead promotes efficient infection in trans of cells that express CD4 and chemokine receptors. We propose that DC-SIGN efficiently captures HIV-1 in the periphery and facilitates its transport to secondary lymphoid organs rich in T cells, to enhance infection in trans of these target cells.",
keywords = "CD4 Antigens/physiology, CD4-Positive T-Lymphocytes/cytology, Cell Adhesion Molecules/physiology, Cell Movement, Cells, Cultured, Cervix Uteri/cytology, Coculture Techniques, Dendritic Cells/immunology, Female, HIV Envelope Protein gp120/metabolism, HIV Infections/transmission, HIV-1/physiology, Humans, Lectins/physiology, Lymph Nodes/cytology, Lymphoid Tissue/cytology, Macromolecular Substances, Male, Mucous Membrane/cytology, Receptors, CCR5/physiology, Receptors, HIV/physiology, Rectum/cytology, Transfection, Uterus/cytology",
author = "Geijtenbeek, {T B} and Kwon, {D S} and R Torensma and {van Vliet}, {S J} and {van Duijnhoven}, {G C} and J Middel and Cornelissen, {I L} and Nottet, {H S} and KewalRamani, {V N} and Littman, {D R} and Figdor, {C G} and {van Kooyk}, Y",
year = "2000",
month = "3",
day = "3",
language = "English",
volume = "100",
pages = "587--97",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",

}

Geijtenbeek, TB, Kwon, DS, Torensma, R, van Vliet, SJ, van Duijnhoven, GC, Middel, J, Cornelissen, IL, Nottet, HS, KewalRamani, VN, Littman, DR, Figdor, CG & van Kooyk, Y 2000, 'DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells' Cell, vol. 100, no. 5, pp. 587-97.

DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. / Geijtenbeek, T B; Kwon, D S; Torensma, R; van Vliet, S J; van Duijnhoven, G C; Middel, J; Cornelissen, I L; Nottet, H S; KewalRamani, V N; Littman, D R; Figdor, C G; van Kooyk, Y.

In: Cell, Vol. 100, No. 5, 03.03.2000, p. 587-97.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells

AU - Geijtenbeek, T B

AU - Kwon, D S

AU - Torensma, R

AU - van Vliet, S J

AU - van Duijnhoven, G C

AU - Middel, J

AU - Cornelissen, I L

AU - Nottet, H S

AU - KewalRamani, V N

AU - Littman, D R

AU - Figdor, C G

AU - van Kooyk, Y

PY - 2000/3/3

Y1 - 2000/3/3

N2 - Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of a DC-specific C-type lectin, DC-SIGN, that is highly expressed on DC present in mucosal tissues and binds to the HIV-1 envelope glycoprotein gp120. DC-SIGN does not function as a receptor for viral entry into DC but instead promotes efficient infection in trans of cells that express CD4 and chemokine receptors. We propose that DC-SIGN efficiently captures HIV-1 in the periphery and facilitates its transport to secondary lymphoid organs rich in T cells, to enhance infection in trans of these target cells.

AB - Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of a DC-specific C-type lectin, DC-SIGN, that is highly expressed on DC present in mucosal tissues and binds to the HIV-1 envelope glycoprotein gp120. DC-SIGN does not function as a receptor for viral entry into DC but instead promotes efficient infection in trans of cells that express CD4 and chemokine receptors. We propose that DC-SIGN efficiently captures HIV-1 in the periphery and facilitates its transport to secondary lymphoid organs rich in T cells, to enhance infection in trans of these target cells.

KW - CD4 Antigens/physiology

KW - CD4-Positive T-Lymphocytes/cytology

KW - Cell Adhesion Molecules/physiology

KW - Cell Movement

KW - Cells, Cultured

KW - Cervix Uteri/cytology

KW - Coculture Techniques

KW - Dendritic Cells/immunology

KW - Female

KW - HIV Envelope Protein gp120/metabolism

KW - HIV Infections/transmission

KW - HIV-1/physiology

KW - Humans

KW - Lectins/physiology

KW - Lymph Nodes/cytology

KW - Lymphoid Tissue/cytology

KW - Macromolecular Substances

KW - Male

KW - Mucous Membrane/cytology

KW - Receptors, CCR5/physiology

KW - Receptors, HIV/physiology

KW - Rectum/cytology

KW - Transfection

KW - Uterus/cytology

M3 - Article

VL - 100

SP - 587

EP - 597

JO - Cell

JF - Cell

SN - 0092-8674

IS - 5

ER -

Geijtenbeek TB, Kwon DS, Torensma R, van Vliet SJ, van Duijnhoven GC, Middel J et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell. 2000 Mar 3;100(5):587-97.