DC-SIGN from African green monkeys is expressed in lymph nodes and mediates infection in trans of simian immunodeficiency virus SIVagm

Mickaël J-Y Ploquin, Ousmane M Diop, Nathalie Sol-Foulon, Lorenzo Mortara, Abdourahmane Faye, Marcelo A Soares, Eric Nerrienet, Roger Le Grand, Yvette Van Kooyk, Ali Amara, Olivier Schwartz, Françoise Barré-Sinoussi, Michaela C Müller-Trutwin

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Abstract

African green monkeys (AGMs) infected by simian immunodeficiency virus (SIV) SIVagm are resistant to AIDS. SIVagm-infected AGMs exhibit levels of viremia similar to those described during pathogenic human immunodeficiency virus type 1 (HIV-1) and SIVmac infections in humans and macaques, respectively, but contain lower viral loads in their lymph nodes. We addressed the potential role of dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN; CD209) in viral dissemination. In previous studies, it has been shown that human DC-SIGN and macaque DC-SIGN allow transmission of HIV and SIVmac to T cells. Here, we looked at the ability of DC-SIGN derived from AGM lymph nodes to interact with SIVagm. We show that DC-SIGN-expressing cells are present mainly in the medulla and often within the cortex and/or paracortex of AGM lymph nodes. We describe the isolation and characterization of at least three isoforms of dc-sign mRNA in lymph nodes of AGMs. The predicted amino acid sequence from the predominant mRNA isoform, DC-SIGNagm1, is 92 and 99% identical to the corresponding human and rhesus macaque DC-SIGN amino acid sequences, respectively. DC-SIGNagm1 is characterized by the lack of the fourth motif in the repeat domain. This deletion was also detected in the dc-sign gene derived from thirteen animals belonging to five other African monkey species and from four macaques (Macaca fascicularis and M. mulatta). Despite three- to seven-amino-acid modifications compared to DC-SIGNmac, DC-SIGNagm1 allows transmission of SIVagm to T cells. Furthermore, AGM monocyte-derived dendritic cells (MDDC) expressed at least 100,000 DC-SIGN molecules and were able to transmit SIVagm to T cells. At a low multiplicity of infection (10(-5) 50% tissue culture infective doses/cell), viral transmission by AGM MDDC was mainly DC-SIGN dependent. The present study reveals that DC-SIGN from a natural host species of SIV has the ability to act as an efficient attachment and transmission factor for SIVagm and suggests the absence of a direct link between this ability and viral load levels in lymph nodes.

Original languageEnglish
Pages (from-to)798-810
Number of pages13
JournalJournal of Virology
Volume78
Issue number2
DOIs
Publication statusPublished - Jan 2004

Cite this

Ploquin, M. J-Y., Diop, O. M., Sol-Foulon, N., Mortara, L., Faye, A., Soares, M. A., ... Müller-Trutwin, M. C. (2004). DC-SIGN from African green monkeys is expressed in lymph nodes and mediates infection in trans of simian immunodeficiency virus SIVagm. Journal of Virology, 78(2), 798-810. https://doi.org/10.1128/JVI.78.2.798-810.2004
Ploquin, Mickaël J-Y ; Diop, Ousmane M ; Sol-Foulon, Nathalie ; Mortara, Lorenzo ; Faye, Abdourahmane ; Soares, Marcelo A ; Nerrienet, Eric ; Le Grand, Roger ; Van Kooyk, Yvette ; Amara, Ali ; Schwartz, Olivier ; Barré-Sinoussi, Françoise ; Müller-Trutwin, Michaela C. / DC-SIGN from African green monkeys is expressed in lymph nodes and mediates infection in trans of simian immunodeficiency virus SIVagm. In: Journal of Virology. 2004 ; Vol. 78, No. 2. pp. 798-810.
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abstract = "African green monkeys (AGMs) infected by simian immunodeficiency virus (SIV) SIVagm are resistant to AIDS. SIVagm-infected AGMs exhibit levels of viremia similar to those described during pathogenic human immunodeficiency virus type 1 (HIV-1) and SIVmac infections in humans and macaques, respectively, but contain lower viral loads in their lymph nodes. We addressed the potential role of dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN; CD209) in viral dissemination. In previous studies, it has been shown that human DC-SIGN and macaque DC-SIGN allow transmission of HIV and SIVmac to T cells. Here, we looked at the ability of DC-SIGN derived from AGM lymph nodes to interact with SIVagm. We show that DC-SIGN-expressing cells are present mainly in the medulla and often within the cortex and/or paracortex of AGM lymph nodes. We describe the isolation and characterization of at least three isoforms of dc-sign mRNA in lymph nodes of AGMs. The predicted amino acid sequence from the predominant mRNA isoform, DC-SIGNagm1, is 92 and 99{\%} identical to the corresponding human and rhesus macaque DC-SIGN amino acid sequences, respectively. DC-SIGNagm1 is characterized by the lack of the fourth motif in the repeat domain. This deletion was also detected in the dc-sign gene derived from thirteen animals belonging to five other African monkey species and from four macaques (Macaca fascicularis and M. mulatta). Despite three- to seven-amino-acid modifications compared to DC-SIGNmac, DC-SIGNagm1 allows transmission of SIVagm to T cells. Furthermore, AGM monocyte-derived dendritic cells (MDDC) expressed at least 100,000 DC-SIGN molecules and were able to transmit SIVagm to T cells. At a low multiplicity of infection (10(-5) 50{\%} tissue culture infective doses/cell), viral transmission by AGM MDDC was mainly DC-SIGN dependent. The present study reveals that DC-SIGN from a natural host species of SIV has the ability to act as an efficient attachment and transmission factor for SIVagm and suggests the absence of a direct link between this ability and viral load levels in lymph nodes.",
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Ploquin, MJ-Y, Diop, OM, Sol-Foulon, N, Mortara, L, Faye, A, Soares, MA, Nerrienet, E, Le Grand, R, Van Kooyk, Y, Amara, A, Schwartz, O, Barré-Sinoussi, F & Müller-Trutwin, MC 2004, 'DC-SIGN from African green monkeys is expressed in lymph nodes and mediates infection in trans of simian immunodeficiency virus SIVagm' Journal of Virology, vol. 78, no. 2, pp. 798-810. https://doi.org/10.1128/JVI.78.2.798-810.2004

DC-SIGN from African green monkeys is expressed in lymph nodes and mediates infection in trans of simian immunodeficiency virus SIVagm. / Ploquin, Mickaël J-Y; Diop, Ousmane M; Sol-Foulon, Nathalie; Mortara, Lorenzo; Faye, Abdourahmane; Soares, Marcelo A; Nerrienet, Eric; Le Grand, Roger; Van Kooyk, Yvette; Amara, Ali; Schwartz, Olivier; Barré-Sinoussi, Françoise; Müller-Trutwin, Michaela C.

In: Journal of Virology, Vol. 78, No. 2, 01.2004, p. 798-810.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - DC-SIGN from African green monkeys is expressed in lymph nodes and mediates infection in trans of simian immunodeficiency virus SIVagm

AU - Ploquin, Mickaël J-Y

AU - Diop, Ousmane M

AU - Sol-Foulon, Nathalie

AU - Mortara, Lorenzo

AU - Faye, Abdourahmane

AU - Soares, Marcelo A

AU - Nerrienet, Eric

AU - Le Grand, Roger

AU - Van Kooyk, Yvette

AU - Amara, Ali

AU - Schwartz, Olivier

AU - Barré-Sinoussi, Françoise

AU - Müller-Trutwin, Michaela C

PY - 2004/1

Y1 - 2004/1

N2 - African green monkeys (AGMs) infected by simian immunodeficiency virus (SIV) SIVagm are resistant to AIDS. SIVagm-infected AGMs exhibit levels of viremia similar to those described during pathogenic human immunodeficiency virus type 1 (HIV-1) and SIVmac infections in humans and macaques, respectively, but contain lower viral loads in their lymph nodes. We addressed the potential role of dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN; CD209) in viral dissemination. In previous studies, it has been shown that human DC-SIGN and macaque DC-SIGN allow transmission of HIV and SIVmac to T cells. Here, we looked at the ability of DC-SIGN derived from AGM lymph nodes to interact with SIVagm. We show that DC-SIGN-expressing cells are present mainly in the medulla and often within the cortex and/or paracortex of AGM lymph nodes. We describe the isolation and characterization of at least three isoforms of dc-sign mRNA in lymph nodes of AGMs. The predicted amino acid sequence from the predominant mRNA isoform, DC-SIGNagm1, is 92 and 99% identical to the corresponding human and rhesus macaque DC-SIGN amino acid sequences, respectively. DC-SIGNagm1 is characterized by the lack of the fourth motif in the repeat domain. This deletion was also detected in the dc-sign gene derived from thirteen animals belonging to five other African monkey species and from four macaques (Macaca fascicularis and M. mulatta). Despite three- to seven-amino-acid modifications compared to DC-SIGNmac, DC-SIGNagm1 allows transmission of SIVagm to T cells. Furthermore, AGM monocyte-derived dendritic cells (MDDC) expressed at least 100,000 DC-SIGN molecules and were able to transmit SIVagm to T cells. At a low multiplicity of infection (10(-5) 50% tissue culture infective doses/cell), viral transmission by AGM MDDC was mainly DC-SIGN dependent. The present study reveals that DC-SIGN from a natural host species of SIV has the ability to act as an efficient attachment and transmission factor for SIVagm and suggests the absence of a direct link between this ability and viral load levels in lymph nodes.

AB - African green monkeys (AGMs) infected by simian immunodeficiency virus (SIV) SIVagm are resistant to AIDS. SIVagm-infected AGMs exhibit levels of viremia similar to those described during pathogenic human immunodeficiency virus type 1 (HIV-1) and SIVmac infections in humans and macaques, respectively, but contain lower viral loads in their lymph nodes. We addressed the potential role of dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN; CD209) in viral dissemination. In previous studies, it has been shown that human DC-SIGN and macaque DC-SIGN allow transmission of HIV and SIVmac to T cells. Here, we looked at the ability of DC-SIGN derived from AGM lymph nodes to interact with SIVagm. We show that DC-SIGN-expressing cells are present mainly in the medulla and often within the cortex and/or paracortex of AGM lymph nodes. We describe the isolation and characterization of at least three isoforms of dc-sign mRNA in lymph nodes of AGMs. The predicted amino acid sequence from the predominant mRNA isoform, DC-SIGNagm1, is 92 and 99% identical to the corresponding human and rhesus macaque DC-SIGN amino acid sequences, respectively. DC-SIGNagm1 is characterized by the lack of the fourth motif in the repeat domain. This deletion was also detected in the dc-sign gene derived from thirteen animals belonging to five other African monkey species and from four macaques (Macaca fascicularis and M. mulatta). Despite three- to seven-amino-acid modifications compared to DC-SIGNmac, DC-SIGNagm1 allows transmission of SIVagm to T cells. Furthermore, AGM monocyte-derived dendritic cells (MDDC) expressed at least 100,000 DC-SIGN molecules and were able to transmit SIVagm to T cells. At a low multiplicity of infection (10(-5) 50% tissue culture infective doses/cell), viral transmission by AGM MDDC was mainly DC-SIGN dependent. The present study reveals that DC-SIGN from a natural host species of SIV has the ability to act as an efficient attachment and transmission factor for SIVagm and suggests the absence of a direct link between this ability and viral load levels in lymph nodes.

KW - Amino Acid Sequence

KW - Animals

KW - Cell Adhesion Molecules/chemistry

KW - Cell Line

KW - Cercopithecus aethiops

KW - Dendritic Cells/metabolism

KW - HeLa Cells

KW - Humans

KW - Lectins, C-Type/chemistry

KW - Lymph Nodes/metabolism

KW - Molecular Sequence Data

KW - Monocytes/metabolism

KW - Receptors, Cell Surface/chemistry

KW - Sequence Alignment

KW - Sequence Analysis, DNA

KW - Simian Acquired Immunodeficiency Syndrome/physiopathology

KW - Simian Immunodeficiency Virus/physiology

U2 - 10.1128/JVI.78.2.798-810.2004

DO - 10.1128/JVI.78.2.798-810.2004

M3 - Article

VL - 78

SP - 798

EP - 810

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 2

ER -