Decreased smooth muscle cell/extracellular matrix ratio of media of femoral artery in patients with atherosclerosis and hyperhomocysteinemia

E G Vermeulen, H W Niessen, M Bogels, C D Stehouwer, J A Rauwerda, V W van Hinsbergh

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The aim of this study was to determine whether the morphology of the muscular femoral artery in patients with atherosclerosis and hyperhomocysteinemia differs from that of atherosclerotic vessels from patients with normal homocysteine levels. Whole-vessel biopsies of the superficial femoral artery were taken from patients with symptomatic atherosclerotic disease with and without hyperhomocysteinemia and from patients without atherosclerosis from traumatic amputations. The morphology of these specimens was studied qualitatively by light and electron microscopy and quantitatively by light microscopy in combination with a video overlay system. Atherosclerotic lesions in patients with hyperhomocysteinemia were morphologically similar to those in patients with normal homocysteine levels, except for a significantly decreased smooth muscle cell/extracellular matrix ratio of the media in hyperhomocysteinemic patients (P=0.02 versus normohomocysteinemic atherosclerotic group and P=0.001 versus group without a history of cardiovascular disease). Hyperhomocysteinemia is associated with a significant decrease of the smooth muscle cell/extracellular matrix ratio of the media of muscular femoral arteries without significant changes in medial thickness. Further investigations should concentrate on the cause of this newly discovered phenomenon and its impact on vascular compliance.

Original languageEnglish
Pages (from-to)573-7
Number of pages5
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume21
Issue number4
Publication statusPublished - Apr 2001

Cite this

@article{116bd06a2da54daca88db4cab0e810d7,
title = "Decreased smooth muscle cell/extracellular matrix ratio of media of femoral artery in patients with atherosclerosis and hyperhomocysteinemia",
abstract = "The aim of this study was to determine whether the morphology of the muscular femoral artery in patients with atherosclerosis and hyperhomocysteinemia differs from that of atherosclerotic vessels from patients with normal homocysteine levels. Whole-vessel biopsies of the superficial femoral artery were taken from patients with symptomatic atherosclerotic disease with and without hyperhomocysteinemia and from patients without atherosclerosis from traumatic amputations. The morphology of these specimens was studied qualitatively by light and electron microscopy and quantitatively by light microscopy in combination with a video overlay system. Atherosclerotic lesions in patients with hyperhomocysteinemia were morphologically similar to those in patients with normal homocysteine levels, except for a significantly decreased smooth muscle cell/extracellular matrix ratio of the media in hyperhomocysteinemic patients (P=0.02 versus normohomocysteinemic atherosclerotic group and P=0.001 versus group without a history of cardiovascular disease). Hyperhomocysteinemia is associated with a significant decrease of the smooth muscle cell/extracellular matrix ratio of the media of muscular femoral arteries without significant changes in medial thickness. Further investigations should concentrate on the cause of this newly discovered phenomenon and its impact on vascular compliance.",
keywords = "Adult, Aged, Biopsy, Cardiovascular Diseases, Comorbidity, Endothelium, Vascular, Extracellular Matrix, Female, Femoral Artery, Homocysteine, Humans, Hyperhomocysteinemia, Immunohistochemistry, Male, Middle Aged, Muscle, Smooth, Vascular, Peripheral Vascular Diseases, Risk Factors, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",
author = "Vermeulen, {E G} and Niessen, {H W} and M Bogels and Stehouwer, {C D} and Rauwerda, {J A} and {van Hinsbergh}, {V W}",
year = "2001",
month = "4",
language = "English",
volume = "21",
pages = "573--7",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

Decreased smooth muscle cell/extracellular matrix ratio of media of femoral artery in patients with atherosclerosis and hyperhomocysteinemia. / Vermeulen, E G; Niessen, H W; Bogels, M; Stehouwer, C D; Rauwerda, J A; van Hinsbergh, V W.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 21, No. 4, 04.2001, p. 573-7.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Decreased smooth muscle cell/extracellular matrix ratio of media of femoral artery in patients with atherosclerosis and hyperhomocysteinemia

AU - Vermeulen, E G

AU - Niessen, H W

AU - Bogels, M

AU - Stehouwer, C D

AU - Rauwerda, J A

AU - van Hinsbergh, V W

PY - 2001/4

Y1 - 2001/4

N2 - The aim of this study was to determine whether the morphology of the muscular femoral artery in patients with atherosclerosis and hyperhomocysteinemia differs from that of atherosclerotic vessels from patients with normal homocysteine levels. Whole-vessel biopsies of the superficial femoral artery were taken from patients with symptomatic atherosclerotic disease with and without hyperhomocysteinemia and from patients without atherosclerosis from traumatic amputations. The morphology of these specimens was studied qualitatively by light and electron microscopy and quantitatively by light microscopy in combination with a video overlay system. Atherosclerotic lesions in patients with hyperhomocysteinemia were morphologically similar to those in patients with normal homocysteine levels, except for a significantly decreased smooth muscle cell/extracellular matrix ratio of the media in hyperhomocysteinemic patients (P=0.02 versus normohomocysteinemic atherosclerotic group and P=0.001 versus group without a history of cardiovascular disease). Hyperhomocysteinemia is associated with a significant decrease of the smooth muscle cell/extracellular matrix ratio of the media of muscular femoral arteries without significant changes in medial thickness. Further investigations should concentrate on the cause of this newly discovered phenomenon and its impact on vascular compliance.

AB - The aim of this study was to determine whether the morphology of the muscular femoral artery in patients with atherosclerosis and hyperhomocysteinemia differs from that of atherosclerotic vessels from patients with normal homocysteine levels. Whole-vessel biopsies of the superficial femoral artery were taken from patients with symptomatic atherosclerotic disease with and without hyperhomocysteinemia and from patients without atherosclerosis from traumatic amputations. The morphology of these specimens was studied qualitatively by light and electron microscopy and quantitatively by light microscopy in combination with a video overlay system. Atherosclerotic lesions in patients with hyperhomocysteinemia were morphologically similar to those in patients with normal homocysteine levels, except for a significantly decreased smooth muscle cell/extracellular matrix ratio of the media in hyperhomocysteinemic patients (P=0.02 versus normohomocysteinemic atherosclerotic group and P=0.001 versus group without a history of cardiovascular disease). Hyperhomocysteinemia is associated with a significant decrease of the smooth muscle cell/extracellular matrix ratio of the media of muscular femoral arteries without significant changes in medial thickness. Further investigations should concentrate on the cause of this newly discovered phenomenon and its impact on vascular compliance.

KW - Adult

KW - Aged

KW - Biopsy

KW - Cardiovascular Diseases

KW - Comorbidity

KW - Endothelium, Vascular

KW - Extracellular Matrix

KW - Female

KW - Femoral Artery

KW - Homocysteine

KW - Humans

KW - Hyperhomocysteinemia

KW - Immunohistochemistry

KW - Male

KW - Middle Aged

KW - Muscle, Smooth, Vascular

KW - Peripheral Vascular Diseases

KW - Risk Factors

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 21

SP - 573

EP - 577

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 4

ER -