Design, synthesis and evaluation of an NLRP3 inhibitor diazirine photoaffinity probe

James R. Hill*, Rebecca C. Coll, Kate Schroder, Avril A. B. Robertson

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


The NLRP3 inhibitor MCC950/CRID3 ameliorates a remarkable number of inflammatory disorders in animal models. Herein we describe a trifluoromethyl phenyl diazirine (TPD) photoaffinity probe, called TPD-950-Br, to probe the molecular interactions of MCC950. We show that TPD-950-Br covalently captures proximal species upon photo-activation and inhibits IL-1β production in an NLRP3 inhibitor assay.
Original languageEnglish
Article number151849
JournalTetrahedron Letters
Issue number19
Publication statusPublished - 7 May 2020
Externally publishedYes

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