Determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids by reversed-phase high pressure liquid chromatography

E. H.H. Wiltink; P. Stekkinger; J. A.C. Brakenhoff; S. A. Danner

doi:10.1007/BF01953628

Determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids by reversed-phase high pressure liquid chromatography

E. H.H. Wiltink^*, P. Stekkinger, J. A.C. Brakenhoff, S. A. Danner

^*Corresponding author for this work

Internal medicine

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In this article a simple method for the determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids is presented, based on reversed-phase ion-pair HPLC with UV detection at 254 nm. No complicated extraction procedure is needed. The stationary phase consists of a reversed-phase C18 stainless steel column and the mobile phase of 0.005 M sodium acetate and 0.0025 M pentanesulfonic acid sodium salt (PIC-B5) as an ion-pair reagent in water (pH 6.5). DHPG is a new antiviral drug in research of the treatment of cytomegalovirus infections. As a pilot study serum and urine samples of a few patients were investigated to gain an impression of the therapeutic range of DHPG. The method is suitable for routine assay of DHPG.

Original language	English
Pages (from-to)	261-264
Number of pages	4
Journal	Pharmaceutisch Weekblad Scientific Edition
Volume	9
Issue number	5
DOIs	https://doi.org/10.1007/BF01953628
Publication status	Published - Oct 1987

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title = "Determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids by reversed-phase high pressure liquid chromatography",

abstract = "In this article a simple method for the determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids is presented, based on reversed-phase ion-pair HPLC with UV detection at 254 nm. No complicated extraction procedure is needed. The stationary phase consists of a reversed-phase C18 stainless steel column and the mobile phase of 0.005 M sodium acetate and 0.0025 M pentanesulfonic acid sodium salt (PIC-B5) as an ion-pair reagent in water (pH 6.5). DHPG is a new antiviral drug in research of the treatment of cytomegalovirus infections. As a pilot study serum and urine samples of a few patients were investigated to gain an impression of the therapeutic range of DHPG. The method is suitable for routine assay of DHPG.",

keywords = "9-(1,3-Dihydroxy-2-propoxymethyl)guanine, Chromatography, high pressure liquid, Detection, ultraviolet, Serum, Urine",

author = "Wiltink, {E. H.H.} and P. Stekkinger and Brakenhoff, {J. A.C.} and Danner, {S. A.}",

year = "1987",

month = oct,

doi = "10.1007/BF01953628",

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journal = "Pharmaceutisch Weekblad - Scientific Edition",

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AU - Wiltink, E. H.H.

AU - Stekkinger, P.

AU - Brakenhoff, J. A.C.

AU - Danner, S. A.

PY - 1987/10

Y1 - 1987/10

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KW - 9-(1,3-Dihydroxy-2-propoxymethyl)guanine

KW - Chromatography, high pressure liquid

KW - Detection, ultraviolet

KW - Serum

KW - Urine

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Determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids by reversed-phase high pressure liquid chromatography

Abstract

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