Abstract
For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.
| Original language | English |
|---|---|
| Pages (from-to) | 493-504 |
| Number of pages | 12 |
| Journal | Immunity |
| Volume | 7 |
| Issue number | 4 |
| Publication status | Published - Oct 1997 |
Cite this
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Developing lymph nodes collect CD4+CD3- LTbeta+ cells that can differentiate to APC, NK cells, and follicular cells but not T or B cells. / Mebius, R E; Rennert, P; Weissman, I L.
In: Immunity, Vol. 7, No. 4, 10.1997, p. 493-504.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Developing lymph nodes collect CD4+CD3- LTbeta+ cells that can differentiate to APC, NK cells, and follicular cells but not T or B cells
AU - Mebius, R E
AU - Rennert, P
AU - Weissman, I L
PY - 1997/10
Y1 - 1997/10
N2 - For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.
AB - For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.
KW - Animals
KW - Animals, Newborn
KW - Antigen-Presenting Cells/cytology
KW - B-Lymphocytes/cytology
KW - CD3 Complex/metabolism
KW - CD4-Positive T-Lymphocytes/cytology
KW - Cell Adhesion Molecules
KW - Cytotoxicity, Immunologic
KW - Fluorescent Antibody Technique, Indirect
KW - GTP-Binding Proteins/genetics
KW - Gene Expression
KW - Histocompatibility Antigens Class II/metabolism
KW - Immunity, Cellular
KW - Immunoglobulins/metabolism
KW - Integrins/metabolism
KW - Interleukin-2/pharmacology
KW - Killer Cells, Natural/cytology
KW - Leukocyte Common Antigens/analysis
KW - Leukopoiesis
KW - Lymph Nodes/cytology
KW - Lymphocyte Subsets/cytology
KW - Lymphotoxin-alpha/metabolism
KW - Lymphotoxin-beta
KW - Membrane Proteins/metabolism
KW - Mice
KW - Mice, Inbred AKR
KW - Mice, Inbred BALB C
KW - Mice, Inbred C57BL
KW - Mucoproteins/metabolism
KW - RNA, Messenger/genetics
KW - Receptors, CXCR5
KW - Receptors, Chemokine
KW - Receptors, Cytokine/genetics
KW - Spleen/embryology
KW - T-Lymphocytes/cytology
M3 - Article
VL - 7
SP - 493
EP - 504
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 4
ER -