Developing lymph nodes collect CD4+CD3- LTbeta+ cells that can differentiate to APC, NK cells, and follicular cells but not T or B cells

R E Mebius, P Rennert, I L Weissman

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.

LanguageEnglish
Pages493-504
Number of pages12
JournalImmunity
Volume7
Issue number4
Publication statusPublished - Oct 1997

Cite this

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title = "Developing lymph nodes collect CD4+CD3- LTbeta+ cells that can differentiate to APC, NK cells, and follicular cells but not T or B cells",
abstract = "For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.",
keywords = "Animals, Animals, Newborn, Antigen-Presenting Cells/cytology, B-Lymphocytes/cytology, CD3 Complex/metabolism, CD4-Positive T-Lymphocytes/cytology, Cell Adhesion Molecules, Cytotoxicity, Immunologic, Fluorescent Antibody Technique, Indirect, GTP-Binding Proteins/genetics, Gene Expression, Histocompatibility Antigens Class II/metabolism, Immunity, Cellular, Immunoglobulins/metabolism, Integrins/metabolism, Interleukin-2/pharmacology, Killer Cells, Natural/cytology, Leukocyte Common Antigens/analysis, Leukopoiesis, Lymph Nodes/cytology, Lymphocyte Subsets/cytology, Lymphotoxin-alpha/metabolism, Lymphotoxin-beta, Membrane Proteins/metabolism, Mice, Mice, Inbred AKR, Mice, Inbred BALB C, Mice, Inbred C57BL, Mucoproteins/metabolism, RNA, Messenger/genetics, Receptors, CXCR5, Receptors, Chemokine, Receptors, Cytokine/genetics, Spleen/embryology, T-Lymphocytes/cytology",
author = "Mebius, {R E} and P Rennert and Weissman, {I L}",
year = "1997",
month = "10",
language = "English",
volume = "7",
pages = "493--504",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "4",

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Developing lymph nodes collect CD4+CD3- LTbeta+ cells that can differentiate to APC, NK cells, and follicular cells but not T or B cells. / Mebius, R E; Rennert, P; Weissman, I L.

In: Immunity, Vol. 7, No. 4, 10.1997, p. 493-504.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Developing lymph nodes collect CD4+CD3- LTbeta+ cells that can differentiate to APC, NK cells, and follicular cells but not T or B cells

AU - Mebius, R E

AU - Rennert, P

AU - Weissman, I L

PY - 1997/10

Y1 - 1997/10

N2 - For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.

AB - For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.

KW - Animals

KW - Animals, Newborn

KW - Antigen-Presenting Cells/cytology

KW - B-Lymphocytes/cytology

KW - CD3 Complex/metabolism

KW - CD4-Positive T-Lymphocytes/cytology

KW - Cell Adhesion Molecules

KW - Cytotoxicity, Immunologic

KW - Fluorescent Antibody Technique, Indirect

KW - GTP-Binding Proteins/genetics

KW - Gene Expression

KW - Histocompatibility Antigens Class II/metabolism

KW - Immunity, Cellular

KW - Immunoglobulins/metabolism

KW - Integrins/metabolism

KW - Interleukin-2/pharmacology

KW - Killer Cells, Natural/cytology

KW - Leukocyte Common Antigens/analysis

KW - Leukopoiesis

KW - Lymph Nodes/cytology

KW - Lymphocyte Subsets/cytology

KW - Lymphotoxin-alpha/metabolism

KW - Lymphotoxin-beta

KW - Membrane Proteins/metabolism

KW - Mice

KW - Mice, Inbred AKR

KW - Mice, Inbred BALB C

KW - Mice, Inbred C57BL

KW - Mucoproteins/metabolism

KW - RNA, Messenger/genetics

KW - Receptors, CXCR5

KW - Receptors, Chemokine

KW - Receptors, Cytokine/genetics

KW - Spleen/embryology

KW - T-Lymphocytes/cytology

M3 - Article

VL - 7

SP - 493

EP - 504

JO - Immunity

T2 - Immunity

JF - Immunity

SN - 1074-7613

IS - 4

ER -