Development of an In Vitro Method to Estimate the Sensitization Induction Level of Contact Allergens

Valentina Galbiati, Sue Gibbs, Erwin Roggen, Emanuela Corsini

Research output: Contribution to journalArticleAcademicpeer-review


Recently, an in vitro procedure, which combines the epidermal equivalent potency assay with assessment of IL-18 to provide a single test for identification and classification of skin sensitizers, was developed and validated. This unit will describe a simple in vitro method for estimation of the expected sensitization induction level interpolating in vitro EC50 and IL-18 SI2 values to predict LLNA EC3 and/or human NOEL from standards curves generated using reference contact allergens, based on the use of Reconstituted human Epidermis (RhE).© 2018 by John Wiley & Sons, Inc.
Original languageEnglish
Pages (from-to)20.15.1-20.15.20
JournalCurrent protocols in toxicology
Publication statusPublished - 2018

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